LED激发光源叶绿素荧光参数在线监控系统

研究设计了一种基于MINIPAM荧光仪调制脉冲式荧光检测技术的LED阵列输出光强叶绿素荧光参数在线监控系统。该系统利用可编程电源为LED列阵提供恒流输出驱动，利用LED列阵作为激发叶绿素产生荧光的光化光，利用MINIPAM荧光仪对植物光系统II的稳态荧光Fs、光适应下最大荧光Fm'进行检测，并结合系统传感器对环境温度、光合有效辐射PAR等植物环境因子的测量，综合分析环境因素对实际量子效率ΦpsII等参数的影响。系统通过串口与上位机通信，上位机可利用DELPHI7语言编制的监控软件对植物光适应下的荧光参数进行实时在线监控。试验表明，与传统的试验方法相比该系统具有精度高、应用范围广等优点，是一种具有广泛应用前景的实时光合能力测量与控制仪器。

Online monitoring system for chlorophyll fluorescence parameters using LED excitation

A new in-situ monitoring system for chlorophyll fluorescence is proposed, using light-emitting diode (LED) excitation by means of the Pulse-amplitude-modulation (PAM) fluorescence detection technology. The system takes programmable power supply as drive power of LED array illumination with high sensitivity and signal-to-noise ratio. The system uses MINIPAM to measure fluorescence parameters such as Fs and Fm'. With the detection of temperature and PAR, and the measurement of other environmental factors, comprehensive analysis of the relationship between environmental factors and the chlorophyll fluorescence parameters such as the impact was conducted. The upper PC can control the power supply and process the data received from MINIPAM by software which programmed in Delphi7. The system takes the monitoring software which was programmed by Delphi7.0 on plants under the fluorescent light to conduct real-time online monitoring of parameters. The results show that, comparing with traditional method, the system has a lot of advantages such as high accuracy and good reliability and convenience. It will be a practical measurement and control system for photosynthesis ability and have a wide application foreground.