盐胁迫下解盐促生菌对棉花种子发芽过程的影响

棉种经解盐促生菌Rs15-4处理后，播于含0.7％NaCl溶液的珍珠岩基质中培养。在种子萌发的过程中测定了细胞膜透性、游离脯氨酸、可溶性蛋白质及可溶性糖的变化。结果表明通过菌株处理后棉花子叶细胞膜透性比对照下降了26.15%；可溶性蛋白质含量高于对照的29.30%；在第7 d脯氨酸含量比对照提高了1.16倍；可溶性糖在发芽的第2 d至第6 d平均高于对照10%左右，以后又趋于稳定。同时，测定了过氧化氢酶(CAT)、过氧化物酶(POD）和超氧化物歧化酶(SOD)的活性，结果表明：解盐促生菌处理后的棉花子叶中CAT和POD活性高峰值较对照提前出现；SOD酶活性大大高于对照。经解盐促生菌Rs15-4处理后，发芽率比对照提高了11.8%。

Effect of relieving-salt-stress and growth-promoting bacteria on germination of cotton seed under salt stress

The cotton seeds treated by Rs15-4 strain of relieving-salt-stress and promoting growth were sowed in vermiculite that contained the NaCl solution(0.7%). The changes of the cell membrane permeability, the contents of free proline, soluble protein, and soluble sugar of cotton cotyledon were determined during germination. The results showed that the cell membrane permeability of cotton seed treated by Rs15-4 strain was 26.15% lower than that of the control; the soluble protein content was obviously 29.30% higher than that of the control; the content of free proline enhanced 1.16 times compared to the control at the 7th day; the soluble sugar content was 10% higher than that of the control from the 2nd day to the 6th day, and tended to stabilization in cotton seeds cotyledon. At the same time, the activities of the catalase(CAT), the peroxidase(POD) and the superoxide dismutase(SOD) were determined. The results indicated that the peak value of activities of POD and CAT of cottonseeds treated by Rs15-4 strain appeared beforehand comparing with the control; the activity of SOD was greatly higher than the control. The germinating rate of cotton seeds treated by Rs15-4 strain was enhanced by 11.8%.