竹子蒸汽爆破法预处理及酶解获取可发酵单糖

竹子的生物量很高,可作为生物能源生产的重要原料.预处理是纤维素乙醇生产中的关键技术,该文以孝顺竹和大木竹为原料,采用蒸汽爆破法对竹子进行预处理,并进行酶解试验,用高效液相色谱法测定预处理滤液和酶解液中的可发酵单糖含量.结果表明,采用0.5％稀硫酸预浸泡能使总糖得率提高49％；汽爆强度对预处理效果有显著影响,汽爆强度指数为3.35时,预处理后固形物的酶解转化率最高；汽爆强度指数为3.65时总糖得率最高,每千克干基竹粉可得单糖289.5 9.汽爆法预处理存在聚糖分解后进入滤液、单糖分解等现象,通过质量平衡才能准确预测其单糖产率.汽爆预处理后竹子的酶解率不及玉米秸秆等生物质原料,可能与其木质化程度高、微观组织结构更为致密等因素有关.

Steam exploded pretreatment and enzymatic hydrolysis of bamboo for fermentable monosaccharides

Bamboo is a kind of high biomass plants. It can be used as important raw material for bio-energy production. Pretreatment is the key technology for the production of cellulosic ethanol. Enzymatic hydrolysis experiments were conducted using the steam exploded pretreated Bambusa multiplex bamboo and Bambusa wenchouensis bamboo. The fermentable sugars were determined from the hydrolyzate and pretreatment filtrate solution by high performance liquid chromatography (HPLC). The results showed that the treatment of pre-soaked in 0.5% sulfuric acid could increase the total sugar yield by 49% compared with non-soaked; the strength of steam exploded pretreatment effected enzymatic conversion significantly, which reached the highest conversion rate of pretreated solids when strength index was 3.35, and the highest total sugars yield obtained when strength index was 3.65. 289.5 g fermentable monosaccharides were got from 1?000 g bamboo of dry basis. It is very important for accurately predicting the yield of sugars from steam exploded pretreated biomass through mass balance because the polysaccharide can be decomposed into the filtrate and the monosaccharide can be decomposed. The hydrolysis conversion of steam exploded treated bamboo is less than corn stover, for the bamboo has more lignin and more dense microstructure.