| 碱性脂肪酶固定化条件及其催化生物柴油的研究 |
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| 引用本文： | 吴茜茜,吴 克,穆文侠,陈 侠.碱性脂肪酶固定化条件及其催化生物柴油的研究[J].农业工程学报,2009,25(9):254-258. |
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| 作者姓名： | 吴茜茜 吴 克 穆文侠 陈 侠 |
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| 作者单位： | 合肥学院生物与环境工程系,合肥,230022 |
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| 基金项目： | 安徽省科技厅科技攻关计划项目（07010202077）；合肥学院应用酶学与工程重点实验室资助 |
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| 摘 要： | 该文以改性的硅胶为载体，通过戊二醛交联固定化碱性脂肪酶，得到较佳固定化条件：当戊二醛浓度为0.8 g/L，给酶量为30 U/g时，酶活回收率效率达到90%以上。通过改变溶剂的种类、给酶量、含水率、底物摩尔比、甲醇的流加方式等参数，考察了脂肪酸和甲醇在固定化碱性脂肪酶催化下合成生物柴油的工艺条件，试验结果表明在20 mL正己烷，给酶量7.5 g（12 U/g），脂肪酸10 g，酸醇摩尔比为1︰1.2，含水率4%条件下，分3次加入甲醇，40℃反应8 h，反应体系酯化率达到了82%。
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| 关 键 词： | 脂肪酶，催化，生物柴油，硅胶，固定化 |
| 收稿时间： | 2008/5/13 0:00:00 |
| 修稿时间： | 2009/6/10 0:00:00 |
Immobilization conditions of alkaline lipase and its application in synthesis of biodiesel |
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| Wu Qianqian,Wu Ke,Mu Wenxia and Chen Xia.Immobilization conditions of alkaline lipase and its application in synthesis of biodiesel[J].Transactions of the Chinese Society of Agricultural Engineering,2009,25(9):254-258. |
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| Authors: | Wu Qianqian Wu Ke Mu Wenxia and Chen Xia |
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| Institution: | Department of Biological and Environmental Engineering, Hefei University, Hefei 230022, China,Department of Biological and Environmental Engineering, Hefei University, Hefei 230022, China,Department of Biological and Environmental Engineering, Hefei University, Hefei 230022, China and Department of Biological and Environmental Engineering, Hefei University, Hefei 230022, China |
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| Abstract: | |
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| Keywords: | lipases catalysis biodiesel modified silica gel immobilization |
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