葡萄糖基质对光合细菌光合产氢过程中菌体活性的影响

研究光合细菌产氢过程中菌体活性的变化及产氢基质对菌体活性的影响对于揭示光合细菌光合产氢本质,提高光合细菌产氢效率具有重要意义。该文以光合细菌为研究对象，以葡萄糖为产氢基质，研究了产氢基质对光合细菌的生长及产氢活性的影响，分析了产氢过程中菌体生长活性下降的原因，并探讨了产氢基质添加浓度对光合细菌吸收光谱的影响。结果发现，光合细菌利用葡萄糖产氢过程中存在着代谢产酸过程，产生的代谢酸引起菌液的酸化，细胞生长受到酸性条件抑制，菌体活性下降。当底物葡萄糖浓度低于3 mmol/L时，光合细菌不产氢，但菌体吸收光谱特征

Influence of glucose substrate on microbial activity of photosynthetic bacteria in process of hydrogen production

Research on microbial activity of photosynthetic bacteria in the process of hydrogen production plays an important role in revealing the nature of hydrogen production and increasing the efficiency of hydrogen production by photosynthetic bacteria.The effects of glucose as carbon substrate on microbial activity of photosynthetic bacteria were researched in this paper,the cause of cell growth activity decline and the influence of glucose on the absorption spectra of photosynthetic bacteria were also analyzed. It showed that in the process of photosynthetic bacteria utilizing glucose to produce hydrogen, glucose was metabolized into organic acid which leads to pH value decreased, and the cell growth activity was inhibited by low pH value. There was no hydrogen produced When the added concentration of glucose below 3 mmol/L, however, it was apparent that the characteristic peaks of absorption spectra and the micrpbial activity was promoted by substrate. When the concentration of glucose was added up to 30 mmol/L, the hydrogen production activity increased significantly, but the characteristic peaks of absorption spectra decreased obviously, and the growth activity was inhibited by substrate. photosynthetic bacteria utilized glucose as substrate to produce hydrogen, the best added concentration of glucose was 180 mmol/L.