黑麂耳成纤维细胞培养及异种重构胚构建

黑麂(Muntiacus crinifrons)是我国特有的珍稀濒危物种。采用组织块贴壁法获得了纯化的黑麂耳成纤维细胞。用3种不同的培养液(DMEM(低糖)、DMEM(高糖)和RPMI-1640)对第3代细胞进行培养,结果细胞群体倍增时间相近,DMEM(低糖)的培养效果稍好。以黑麂耳成纤维细胞为核供体,以山羊和兔卵母细胞为胞质受体,通过核移植构建异种克隆胚,囊胚率分别为0和11.5%,结果表明两种胞质受体均能支持黑麂耳成纤维细胞核的重编程,但重构胚的发育能力有所差别。

Culture of Fibroblast Cells and Interspecies Embryos Reconstruction in Black Muntjac In vitro

The black muntjac (Muntiacus crinifrons) is a critically endangered mammalian species that is confined to a narrow region of southeastern China.The present study cultured the homogeneous ear fibroblast cell of the endangered black muntjac. The population doubling time of subculture cells in three different culture media, DMEM(low glucose), DMEM(high glucose) and RPMI-1640, was not significantly different, but DMEM (low glucose) was more efficient for the growth of the cells. The interspecies embryos were reconstructed using the fibroblasts of black muntjac as donors and enucleated rabbit or goat oocytes as recipients. The blastocyst rates were 0 and 11.5% respectively. The results demonstrated that two cytoplasts could support reprogramming the ear fibroblasts of black muntjac, but the developmental potential of the reconstructed embryos was different.