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| 烟粉虱B型及二个非B型种群的SCAR分子标记 | |
| 引用本文： | 臧连生,江彤,徐婧,刘树生,张友军.烟粉虱B型及二个非B型种群的SCAR分子标记[J].农业生物技术学报,2006,14(2):208-212. |
| 作者姓名： | 臧连生 江彤 徐婧 刘树生 张友军 |
| 作者单位： | 1. 浙江大学应用昆虫学研究所,杭州,310029;吉林农业大学农学院,吉林,130118 2. 浙江大学生物技术研究所,杭州,310029 3. 浙江大学应用昆虫学研究所,杭州,310029 4. 中国农业科学院蔬菜花卉研究所,北京,100081 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 用随机引物H16对浙江采集的烟粉虱(Bemisia tabaci)B型和2个非B型种群进行RAPD分子标记，结果表明: B型、非B型ZHJ-1和非B型ZHJ-2 3个种群分别能稳定地扩增出446、390和1317 bp的特异性片段。将3个种群的特异性片段分别克隆和测序，根据测序结果设计3对SCAR引物。通过PCR反应条件的优化，B型、非B 型ZHJ-1和非B型ZHJ-2 3个种群各自的SCAR引物分别能扩增出本种群的特异性条带，其大小依次为439、366和1238 bp，而其它种群与温室白粉虱(Trialeurodes vaporariorum )则无扩增。 |
| 关 键 词： | 烟粉虱 生物型 种群 RAPD SCAR 分子标记 |
| 文章编号： | 1006-1304（2006）02-0208-06 |
| 收稿时间： | 2005-4-11 |
| 修稿时间： | 2005-07-10 |
SCAR Molecular Markers of the B Biotype and Two Non-B Populations of the Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) |
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| ZANG Lian-sheng,JIANG Tong,XU Jing,LIU Shu-sheng,ZHANG You-jun.SCAR Molecular Markers of the B Biotype and Two Non-B Populations of the Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)[J].Journal of Agricultural Biotechnology,2006,14(2):208-212. | |
| Authors: | ZANG Lian-sheng JIANG Tong XU Jing LIU Shu-sheng ZHANG You-jun |
| Institution: | 1. Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China; 2. College of Agriculture, Jilin Agricultural University, Jilin 130118, China; 3. Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China; 4. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| Abstract: | RAPD was performed with random primer H16 for B biotype and two non-B populations of Bemisia tabaci collected from Zhejiang, China. The results revealed that the specific sequence fragments containing 446, 390 and 1317 nt were amplified for the B biotype, non-B ZHJ-1 and ZHJ-2 populations, respectively. The three specific fragments were cloned and sequenced, and three pairs of SCAR primers were designed according to the sequences. With improvement of the conditions of PCR, the specific fragment of B biotype, non-B ZHJ-1 and non-B ZHJ-2 populations, namely, 439, 366 and 1238 nt, respectively, could be amplified with the SCAR primer of the corresponding population, while the specific fragments of the other populations of B. tabaci or Trialeurodes vaporariorum could not be amplified. |
| Keywords: | Bemisia tabaci biotype population RAPD SCAR molecular markers |
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