猪HUMMLC2B基因第一内含子多态与胴体性状的相关性研究

快肌肌浆球蛋白轻链2（fast skeletal myosin light chain 2,HUMMLC2B或MYLPF）是一种钙结合蛋白，参与多种生命活动，是近几年研究较多的一种肌浆球蛋白。本研究采用PCR-RFLP技术，分析了HUMMLC2B基因第1内含子中的MspⅠ酶切多态（T613C）在7个品种猪中的多态性分布及其与胴体性状和肉质性状间的相关，结果表明：在检测的猪群中除长白猪中A等位基因与B等位基因频率的比例为1：2外，其余的均为B等位基因占绝对优势，且A等位基因纯合个体只在长白猪中检测到。在6个群体中，基因型效应与眼肌宽度、眼肌面积 肌内脂肪和肌内水分等差异极显著（P<0.01），与瘦肉率和皮率等性状差异显著（P<0.05）。最后对该基因在各组织中的表达情况进行了研究；该位点是否用作为改良猪胴体、肉质性状的标记位点并在标记辅助选择中加以应用还有待进一步的研究。

Association of Single Nucleotide Polymorphisms in Intron 1 of Porcine HNMMLC2B Gene with Meat Quality and Carcass Traits

Fast skeletal myosin light chain 2 (HUMMLC2B or MYLPF) is a Ca-binding protein which participate in many vital movements, and it has become a hot spot in the myosin studies in recent years．Using PCR-RFLP，the distributes of MspⅠ polymorphisms (T613C) in intron 1 of HUMMLC2B gene in seven pig populations were analyzed，and the relationship between the HUMMLC2B genotypes and pork quality and carcass traits was evaluated．The results showed the frequency of allele B were predominant in all other breeds except for Landrace, in which the ratio pf allele A and B was 1:2 and the homozygote of allele A was found. In six detected pig populations, very significant effects of this locus on loin eye width, loin eye area, intramuscular and water moisture (P<0.01) were found, and this locus was significantly associated with lean meat percentage and skin percentage (P<0.05). The expression of HUMMLC2B in the tissues also was examined. It needs further studied whether the polymorphic site is to be used as the candidate locus affecting carcass and meat quality traits in markers assisted selection.