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绵羊mtDNA D-环序列与微卫星DNA遗传多样性和系统发育分析
引用本文:成述儒,罗玉柱,韩建林,Olivier Hanotte,胡江.绵羊mtDNA D-环序列与微卫星DNA遗传多样性和系统发育分析[J].农业生物技术学报,2009,17(6):988-994.
作者姓名:成述儒  罗玉柱  韩建林  Olivier Hanotte  胡江
作者单位:1. 甘肃农业大学动物科学技术学院,兰州,730070;甘肃农业大学研究测试中心,兰州,730070
2. 甘肃农业大学动物科学技术学院,兰州,730070;国际家畜研究所,内罗毕,00100,肯尼亚
3. 国际家畜研究所,内罗毕,00100,肯尼亚
基金项目:国家科技支撑计划课题,甘肃省生物技术专项,甘肃省科技厅项目,国际家畜研究所动物遗传资源项目 
摘    要:采用mtDNA D-环序列和微卫星DNA两种标记方法,对9个绵羊群体225只个体进行遗传多样性和系统发育分析。结果表明:两种方法在遗传多样性分析中得出的结论一致,即在研究的9个绵羊群体中青海藏羊的遗传多样性最丰富,而湖羊和岷县黑裘皮羊遗传多样性都较低。但在系统发育分析中,通过mtDNA D-环序列和微卫星DNA座位数据构建系统发育树,结果表现为很大的不同。由于微卫星DNA符合孟德尔遗传规律,能够反映群体间的亲缘关系,构建的系统发育树结构可靠。mtDNA是核外遗传物质,具有母系遗传的特点,在反映群体间的亲缘关系上不具优势。此外,青海细毛羊和甘肃高山细毛羊的育种实践表明它们的遗传来源相似,亲缘关系相近,这与微卫星DNA座位数据构建系统发育树反映的结果一致,因此,在群体的亲缘关系研究上,微卫星DNA数据分析的结果比mtDNA序列分析结果更可信。172个单倍型序列网络关系分析表明研究的9个绵羊群体可能有三个母系起源。

关 键 词:mtDNA  微卫星DNA  遗传多样性  系统发育  绵羊
收稿时间:2008-11-11
修稿时间:2009-1-10

Analysis of Genetic Diversity and Phylogenetics Using mtDNA D-loop Sequence and Microsatellite DNA Marker in Sheep
CHENG Shu-ru,LUO Yu-zhu,HAN Jian-lin,Olivier Hanotte,HU Jiang.Analysis of Genetic Diversity and Phylogenetics Using mtDNA D-loop Sequence and Microsatellite DNA Marker in Sheep[J].Journal of Agricultural Biotechnology,2009,17(6):988-994.
Authors:CHENG Shu-ru  LUO Yu-zhu  HAN Jian-lin  Olivier Hanotte  HU Jiang
Abstract:We analysised the genetic diversity and phylogenesis of 225 individuals of 9 sheep populations using mitochondrial D-loop sequences and microsatellite DNA. The results of the two methods in genetic diversity analysis were consistent. It was that Qinghai Tibetan sheep had rich genetic diversity, but Hu sheep and Minxian Black Fur sheep's genetic diversity were lower than others. While in phylogenesis analysis, phylogenetic trees based on mtDNA D-loop sequence and microsatellite DNA were different. Because microsatellite DNA accords with Mendelian inheritance and can reflect population relationship more reliability. mtDNA is extranuclear inheritance and belongs to maternal inheritance and is not superiority in population relationship analysis. Furthermore, the breeding background of Qinghai Merino and Gansu Alpine Merino showed that they had close relationship. This was accorded with phylogenetic tree constructed by microsatellite DNA. Thus we conclude that microsatellite DNA is more reliable in population relationship analysis. Further network analysis of the phylogenetic relationship of the 172 haplotypes identified from 9 sheep populations supported there are three distinct maternal lineages.
Keywords:mtDNA  mitochondrial DNA  microsatellite DNA  genetic diversity  phyiogenetics  sheep
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