| 稻米深加工产品基因组提取方法及其对PCR的影响 |
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| 引用本文： | 许文涛,黄昆仑,芦云,郭峰,杨蓉,秦伟,罗云波.稻米深加工产品基因组提取方法及其对PCR的影响[J].农业生物技术学报,2007,15(1):97-101. |
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| 作者姓名： | 许文涛 黄昆仑 芦云 郭峰 杨蓉 秦伟 罗云波 |
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| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083;农业部转基因食品检验监督测试中心,北京,100083
2. 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 基金项目： | 引进国际先进农业科技计划(948计划) |
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| 摘 要： | 以4种米粉为原料,每个样品做3个梯度(120、800和2000mg),采用改进的经典酚/仿法、CTAB(十六烷基三乙基溴化铵)沉淀法以及盐酸胍/氯仿法提取基因组,经PCR扩增内标基因(SPS)检测方法的优劣。结果显示,120mg的样品经3种方法提取的基因组均不能扩增出内标基因;800和2000mg的样品只有用CTAB沉淀法提取的基因组(采用相同的模板量)能全部扩增出内标基因。结果表明,CTAB沉淀法提取基因组的效果最好,对PCR的抑制现象最少。
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| 关 键 词： | 稻米深加工产品 基因组 十六烷基三乙基溴化铵沉淀法 |
| 文章编号： | 1006-1304（2007）01-0097-05 |
| 收稿时间： | 2006-05-12 |
| 修稿时间： | 2006-05-122006-07-13 |
Extraction Methods of Genome in Processed Rice and Their Influences on PCR |
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| XU Wen-tao,HUANG Kun-lun,LU Yun,GUO Feng,YANG Rong,QIN Wei,LUO Yun-bo.Extraction Methods of Genome in Processed Rice and Their Influences on PCR[J].Journal of Agricultural Biotechnology,2007,15(1):97-101. |
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| Authors: | XU Wen-tao HUANG Kun-lun LU Yun GUO Feng YANG Rong QIN Wei LUO Yun-bo |
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| Abstract: | Genomic DNA of four kinds of processed rice products were extracted by three methods: classic phenol/chloroform extraction method, CTAB (cetyl trimethyl ammonium bromide) precipitation extraction method and guanidine hydrochloride extraction method. The total DNA was extracted from different starting processed rice products(120,800 and 2 000 mg). The quality and quantity of extracted DNA were assayed by PCR with the primer pair SPS -F/SPS -R. Results indicated that all of extracted genomic DNA by the CTAB precipitation extraction method could be amplified by PCR with exception of 120 mg starting material, showing that CTAB precipitation extraction method has the highest efficiency of extracting genomic DNA and the least inhibiting information on PCR. |
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| Keywords: | PCR |
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