泥蚶能量代谢相关的同工酶的分析

利用聚丙烯酰胺凝胶电泳分析了泥蚶（Tegillarca granosa）能量代谢相关的10种同工酶。酯酶和α-淀粉酶是同能量吸收有关的酶，主要分布于消化腺里。苹果酸脱氢酶、苹果酸酶、异柠檬酸脱氢酶、琥珀酸脱氢酶、醇脱氢酶、乳酸脱氢酶、6-磷酸葡萄糖脱氢酶和三磷酸腺苷激酶是与能量代谢相关的酶。泥蚶的能量供应有3种途径：有氧呼吸、无氧酵解和磷酸戊糖途径。从泥蚶体内含有很高活性的6-磷酸葡萄糖脱氢酶可知，磷酸戊糖途径是泥蚶糖代谢的主要途径，NADP为供能体。同其它贝类相比，泥蚶的三磷酸腺苷激酶的活性很低，这与它们的埋栖生活不活跃运动的习性是一致的。

Analysis of Isozymes Related to Energy Metabolism of Adult Tegillarca granosa

Ten isozymes connected with energy metabolism in Tegillarca granosa were analysed by vertical polyacrylamide gel electrophoresis. Esterase and α-amylase are isozymes related with energy intake, their activities were high in the digestive gland.Malate dehydrogenase, malic enzyme, isocitrate dehydrogenase, succinate dehydrogenase, alcohol dehydrogenase, lactate dehydrogenase, 6-phosphogluconate dehydrogenase (G-6-PDH) and adenosine triphosphatase (ATPase) are isozymes related with energy metabolism. The main energy supply of T. granosa comes from aerobic respiration, anaerobic metabolism and phosphopentase pathway plays the auxiliary role in the energy metabolism. The high activity of G-6-PDH in T. granosa might mean a considerable proportion of carbohydrates metabolized through this pathway. This reaction could provide abundant NADP for the metabolism in T.granosa. Compared with other shellfish, T. granosa had lower activity of ATPase, which might have some relationships with the amnicolous life and weak moving ability of this animal.