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协青早B//协青早B/东乡野生稻BC1F5群体产量性状QTL分析
引用本文:黄得润,陈洁,侯丽娟,樊叶杨,庄杰云.协青早B//协青早B/东乡野生稻BC1F5群体产量性状QTL分析[J].农业生物技术学报,2008,16(6).
作者姓名:黄得润  陈洁  侯丽娟  樊叶杨  庄杰云
作者单位:中国水稻研究所,国家水稻改良中心/水稻生物学国家重点实验室,杭州,310006
基金项目:国家自然科学基金,中国水稻研究所基本科研业务费项目 
摘    要:两年同地种植协青早B//协青早B/东乡野生稻BC1F5群体的202个株系,利用含149个DNA标记的连锁图谱,检测到23个产量性状QTLs,包括每株穗数2个、每穗实粒数4个、每穗总粒数6个、结实率5个、千粒重4个和单株产量2个;有9个QTLs的增效等位基因来自东乡野生稻,包括每株穗数2个、每穗实粒数1个、每穗总粒数5个和千粒重1个,其中6个与前人应用野栽群体检测到的产量性状QTLs位于相似区间。这23个QTLs分布于除第11染色体外的所有水稻染色体中,其中18个形成8个QTL簇,含增效等位基因来自野生稻的2个、来自协青早B的4个,以及效应方向发生变化的2个。这些结果为东乡野生稻增产等位基因的发掘提供了基础。

关 键 词:东乡野生稻  BC1F5群体  产量性状  数量性状座位  增效等位基因
收稿时间:2008-2-25
修稿时间:2008-4-8

Identification of QTLs for Yield Traits in the BC1F5 Population of Xieqingzao B//Xieqingzao B/Dongxiang Wild Rice
HUANG De-run,CHEN Jie,HOU Li-juan,FAN Ye-yang,ZHUANG Jie-yun.Identification of QTLs for Yield Traits in the BC1F5 Population of Xieqingzao B//Xieqingzao B/Dongxiang Wild Rice[J].Journal of Agricultural Biotechnology,2008,16(6).
Authors:HUANG De-run  CHEN Jie  HOU Li-juan  FAN Ye-yang  ZHUANG Jie-yun
Abstract:A rice population consisting of 202 BC1F5 lines of the interspecies cross Xieqingzao B (Oryza sativa) //Xieqingzao B/Dongxiang wild rice (O. rufipogon) was tested in 2 years at the same trial site. A total of 23 QTLs for yield traits were detected using a linkage map consisting of 149 markers. The numbers of QTLs detected for individual traits were 2 for the number of panicle per plant (NP), 4 for the number of filled grains per panicle (NFGP), 6 for the total number of spikelets per panicle (TNSP), 5 for spikelet fertility (SF), 4 for 1000-grain weight (TGWT) and 2 for grain yield per plant (GYD). Nine of the QTLs had the enhancing alleles from the Dongxiang wild rice, including 2 for NP, 1 for NFGP, 5 for TNSP and 1 for TGWT. Six of them were located in intervals where QTLs for yield traits have been reported in other studies using 9 interspecies populations. The 23 QTLs were distributed in all the 12 rice chromosomes except chromosome 11. Eighteen of the QTLs were located in 8 clusters. The enhancing alleles were from Xieqingzao B in 4 clusters and from the Dongxiang wild rice in 2 clusters, while the allelic direction in the remaining clusters varied for different traits.
Keywords:Dongxing wild rice  BC1F5 population  yield traits  quantitative trait loci  enhancing allele
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