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| 抗青枯病转群体感应猝灭基因烟草的培育 | |
| 引用本文： | 刘志静,张争,徐进,许景升,何礼远,易图永,冯洁.抗青枯病转群体感应猝灭基因烟草的培育[J].农业生物技术学报,2008,16(6). |
| 作者姓名： | 刘志静 张争 徐进 许景升 何礼远 易图永 冯洁 |
| 作者单位： | 1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,1000941;湖南农业大学生物安全科学技术学院,长沙,410128 2. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100094 3. 湖南农业大学生物安全科学技术学院,长沙,410128 |
| 基金项目： | 国家科技支撑计划，国家高技术研究发展计划(863计划)，国家自然科学基金，国家重点实验室自主研究课题专项经费资助项目 |
| 摘 要： | 利用具有猝灭青枯菌群体感应信号分子功能的aac基因构建高效植物表达载体，通过农杆菌介导的方法转化烟草，获得了卡那霉素抗性植株57株。经PCR及RT-PCR检测，筛选得到30株阳性转基因再生苗，并证实了目的基因已经整合到烟草基因组中，且在转录水平上得到表达。转基因植株苗期抗青枯病试验表明，16天后转基因烟草较对照植株病情指数降低了51，相对病情指数为56.5％。 |
| 关 键 词： | aac基因 群体猝灭 转基因烟草 抗病性 |
| 收稿时间： | 2008-1-23 |
| 修稿时间： | 2008-3-4 |
Breeding of Transgenic Tobacco with Quorum-quenching Gene from Ralstonia solanacearum and Its Resistance against Bacterial Wilt |
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| LIU Zhi-jing,ZHANG Zheng,XU Jin,XU Jing-sheng,HE Li-yuan,YI Tu-yong,FENG Jie.Breeding of Transgenic Tobacco with Quorum-quenching Gene from Ralstonia solanacearum and Its Resistance against Bacterial Wilt[J].Journal of Agricultural Biotechnology,2008,16(6). | |
| Authors: | LIU Zhi-jing ZHANG Zheng XU Jin XU Jing-sheng HE Li-yuan YI Tu-yong FENG Jie |
| Abstract: | Plant expression vector pBI121/Ω4A/aac was constructed by cloning of aac gene which can quench the quorum-sensing of Ralstonia solanacearum. The aac gene was transferred into tobacco mediated by Agrobacterium tumefaciens. Under Kanamycin selection pressure, fifty-seven resistant lines were obtained. PCR, RT-PCR analysis indicated that aac gene had been integrated in tobacco genome and translated. Inoculation testing showed that the disease index of transgenic tobacco was reduced by 51 in greenhouse after 16 days of inoculation, 56.5 percentage points down from the contrast. |
| Keywords: | aac gene quorum-quenching transgenic tobacco disease resistance |
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