| 猪 前 体 脂 肪 细 胞 的 原 代 培 养 |
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| 引用本文： | 屈长青,张国华,陈粉粉,赵丽丽,杨公社.猪 前 体 脂 肪 细 胞 的 原 代 培 养[J].农业生物技术学报,2005,13(5):649-653. |
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| 作者姓名： | 屈长青 张国华 陈粉粉 赵丽丽 杨公社 |
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| 作者单位： | 西北农林科技大学动物脂肪沉积与肌肉发育实验室,杨凌,712100 |
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| 基金项目： | 国家自然科学基金项目（No.30471239）资助. |
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| 摘 要： | 建立猪前体脂肪细胞原代培养方法，研究猪脂肪组织增生的生物学特征。选用出生7d的仔猪脂肪组织，采用原代消化细胞培养法培养出棱形细胞，该细胞成分均一、生长旺盛和充脂率高。经形态学动态变化观察、MTT比色法、生长曲线及油红O染色提取法测定，证明是功能活跃的前体脂肪细胞，并在体外重现了其增殖的全过程。
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| 关 键 词： | 猪 前体脂肪细胞 细胞培养 脂肪组织 |
| 收稿时间： | 2004-08-26 |
| 修稿时间： | 2004-11-09 |
Primary Culture of Porcine Preadipocyte |
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| QU Chang-Qing,ZHANG Guo-Hua,CHEN Fen-Fen,ZHAO Li-Li,YANG Gong-She.Primary Culture of Porcine Preadipocyte[J].Journal of Agricultural Biotechnology,2005,13(5):649-653. |
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| Authors: | QU Chang-Qing ZHANG Guo-Hua CHEN Fen-Fen ZHAO Li-Li YANG Gong-She |
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| Institution: | Laboratory of Animal Fat Deposition and Muscle Development, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China |
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| Abstract: | A primary porcine preadipoeyte culture method for understanding the properties of hyperplasia of pig adipose tissue was established. The 7-day old crossbred pig was purchased and adipose tissue was removed with sterile instruments from the dorsal neck, shoulder and back regions. Using primary cell culture method, isolated preadipoeyte were cultured. The cells were highly homogeneous and proliferative, and had a high differentiation rate. Their dynamic morphological changes, MTT, growth curve and extracting stained intraeytoplasmie lipid with oil red O, all verified their preadipoeyte identity. Under controlled conditions, the preadipoeytes replayed their hyperplasia process in vitro. In young pig adipose tissue, there were preadipoeytes that could differentiate into mature adipoeytes. |
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| Keywords: | porcine preadipocyte cell culture adipose tissue |
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