中国板栗SSR标记的研究

本研究从中国板栗“艳红”中分离到25个SSR标记。为了鉴定SSR位点，在重复序列的两侧侧翼序列设计引物，并通过化学荧光检测法对6个板栗样品进行检测，共检测到18个多态性位点，每个位点的等位基因数为2~6个。挑选12个位点，通过半自动系统ABI PRISM 377对中国北方24个板栗品种进行分析，这12个标记显示了高达共75个等位基因的遗传多态性，每个位点的等位基因为4~10个，平均为6.3个，预期的平均杂合性为0.743（介于0.680~0.845），检测到的平均杂合性为0.829（介于0.730~0.930）。无效等位基因估算频率显示为3个位点的正向价值，除了一个位点外，这些价值都很低，鉴定机率为7.01×10-11，亲缘关系鉴定机率非常高，为0.999，足够用于花粉流的研究。

Development and Characterization of SSR Markers in Chinese Chestnut (Castanea mollissima)

Twenty-five simple sequence repeat (SSR) markers were isolated and characterized in Castanea mollissima from the cultivar Yanhong. For the identification of SSR loci, primer were designed on each side flanking the repeat region and they were initially tested on 6 chestnut samples using chemiluminesence detection. Eighteen loci where shown to be polymorphic and the number of alleles detected per locus varied from 2 to 6. Twelve loci were chosen for the analysis of 24 cultivars grown in North Chinese using the semi-automatic system ABI PRISM 377. These 12 markers showed a high level of genetic polymorphism with a total of 75 alleles; the number of alleles ranged from 4 to 10 per locus, with an average level of 6.3. The mean expected and observed heterozygosity were 0.743 (range: 0.680－0.845) and 0.829 (range: 0.730－0.930) respectively. The estimated frequence of null alleles showed a positive value for 3 loci, but except for 1 locus, the values were very low. The total value for the probability of identity was 7.01×10-11. Paternity exclusion probability was very high (0.999), sufficiently high to study pollen flow.