猪RBP1和RBP4基因的定位、组织表达谱分析、多态研究及相关分析

类维生素A（维生素A及其代谢产物）在动物的视觉、生长、繁殖、细胞的分化及胚胎发育等方面起重要的作用。利用猪的辐射杂种克隆板，将猪细胞视黄醇结合蛋白基因1（RBP1）和猪血浆视黄醇结合蛋白基因4（RBP4）分别定位在猪13号和14号染色体上。利用半定量RT－PCR方法，对这两个基因在成年五指山猪十二种不同组织（肺、骨骼肌、脾、心脏、胃、大肠、淋巴结、小肠、肝、大脑、肾、脂肪）的组织表达谱进行了分析。在猪的血浆视黄醇结合蛋白基因4中，发现一个单核苷酸多态位点（RBP4-A/G156），并用基于聚合酶链式反应的限制性片段长度多态性方法(PCR-RFLP)，对这一多态在莱芜黑猪、五指山小型猪、贵州小型猪、广西巴马小型猪和通城实验猪群中的分布进行了研究。在通城实验猪群中，进行了不同基因型与性状间的关联分析，发现其不同的基因型与胴体直长、胴体斜长、血红蛋白浓度、平均血细胞血红蛋白量、达上市体重日龄高度相关。在猪的生产和育种中，这一多态位点可能会成为有用的分子标记。

Mapping, Tissue Distribution and Polymorphism of Porcine Retinol Binding Protein Genes (RBP1 and RBP4 )

The retinoid (vitamin A and its derivatives) plays a critical role in vision, growth, reproduction, cell differentiation, and embryonic development. Using the INRA IMpRH panel, porcine cellular retinol binding protein genes 1 (RBP1) and plasma retinol binding protein gene 4 (RBP4) were assigned to porcine chromosomes 13 and 14, respectively. The mRNA distributions of the two genes in adult Wuzhishan pig tissues (lung, skeletal muscle, spleen, heart, stomach, large intestine, lymph code, small intestine, liver, brain, kidney, and fat) were examined. One SNP was identified in PRB4, and analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in Laiwu, Wuzhishan, Guizhou, Bama pig breeds and Tongcheng experimental population. Significant associations were found between different genotypes of RBP4-A/G156 with maximum carcass length (LM), minimum carcass length (LN), hemoglobin (HGB), mean corpuscular volume hemoglobin (MCH), and day to slaughter weight (Age) traits. This SNP locus may be used as the genetic markers in pig breeding and porcine production.