转防治软腐病基因拟南芥的获得及其抗病性研究

AHLs是细菌群体感应中的关键信号分子，参与包括导致作物软腐病的胡萝卜软腐欧文氏菌在内的许多植物病原菌致病因子的表达。AHL内酯酶能降解AHLs使其达不到临界浓度，从而阻断了病原菌的致病过程。将克隆到的AHL内酯酶基因SS10置于CaMV 35S启动子下构建双元载体，通过根癌农杆菌介导转化拟南芥。通过抗性筛选和分离比获得纯合转基因植物种子，进行了PCR及RT-PCR鉴定，证明目的基因在转基因拟南芥中整合并表达。初步抗病性实验表明，部分转基因植株具有显著的抗软腐病能力。

Transgenic Expression in Arabidopsis thaliana of an N-acylhomoserine Lactonase Increases Its Resistance to Soft Rot Disease

N-acylhomoserine lactones (AHLs) serve as signal molecules of bacterial quorum sensing system, regulate virulence of several plant pathogens. The N-acylhomoserine lactonase (AHL-lactonase) hydrolyzes AHLs and attenuates the pathogenicity of plant pathogenic bacteria. A plant binary vector carrying aiiA under the control of 35s promoter was constructed into Arabidopsis thaliana by Agrobacterium mediated transformation. The homozygous transgenic plants seeds were botained by resistance screen. The gene transformation and expression was confirmed by PCR and RT-PCR respectively.The aiiA expressing transgenic A. thaliana showed an increased resistance to soft rot disease caused by Erwinia carotovora subsp.carotovora.