来自斯卑尔脱小麦新的抗条锈病基因YrSp的分子标记定位

摘要： 利用SSR分子标记技术，鉴定抗源来自斯卑尔脱小麦(Triticum spelta L.)的杂交组合分离群体温6/cp90.0.2.4.1(169/T.sp.al //宝丰7228)所携带抗条锈病(Puccinia striiformis Westend f. sp. tritric )基因是否为新基因，并对其进行染色体定位研究。经过对150对微卫星引物的筛选，发现位于3A染色体长臂上的Xgwm155引物所扩主带Xgwm155-147bp 与该基因表现连锁，连锁距离为40.5 cM，说明其位于3A染色体上。由于来自斯卑尔脱小麦的已知抗条锈病基因Yr5位于2B染色体长臂上，研究定位的位于3A染色体上的抗条锈病基因不同于Yr5，暂定名为YrSp。

Molecular Marker Location of a Novel Yellow Rust Resistance Gene YrSp Derived from Spelt Wheat

Abstract: The losing of varietal resistance to yellow rust(Puccinia striiformis Westend f. sp. tritric ) is an important factor causing periodical and massive yellow rust outbreak in wheat growing area. Emergency and development of new isolates of yellow rust pathogen have put widely planted varieties in China into a serious crisis of resistance losing and therefore put searching for new resistance resources into great need. Molecular marker technology could facilitate the identification and location of novel genes. The current study was undertaken using SSR technology in order to identify a novel yellow rust resistance gene derived from Spelt wheat (Triticum spelta L.) and study its chromosomal location. After screening 150 wheat microsatellite primers, a marker Xgwm155-147 bp located on chromosome 3A linked to this gene with the genetic distance of 40.5 cM was found, which indicated that the resistance gene is located on chromosome 3A. Since the only yellow rust resistance gene Yr5 known to be derived from Spelt is located on chromosome 2BL, the resistance gene identified in this study might be a novel yellow rust resistance gene which is temporarily designated YrSp.