烟草扭脉病毒部分基因组特征及其分类地位分析

通过RT-PCR从烟草丛顶病发病烟株总RNA中获得烟草扭脉病毒（Tobacco vein distorting virus，TVDV）基因组部分序列。序列分析显示该片段全长1655bp，共包含TVDV的部分RdRp基因、完整的CP基因和完整的MP基因。其中RdRp基因与CP基因的间隔区（IR）长201nts，符合马铃薯卷叶病毒属病毒的归属依据；对RdRp、CP、MP基因以及IR序列的对比分析，显示了TVDV与黄症病毒科马铃薯卷叶病毒属病毒的较高同源性。根据三个ORF编码的氨基酸序列构建的分子同源树的分析，也都显示了TVDV与黄症病毒科马铃薯卷叶病毒属病毒的同源性最高，进一步证实了TVDV应该是黄症病毒科马铃薯卷叶病毒属的确定成员。

Analysis of Partial Genome Structure of Tobacco vein distorting virus and Its Classification

Partial genome of Tobacco vein distorting virus（TVDV）was amplified from total RNA extracted from tobacco plants infected with tobacco bushy top disease by RT-PCR. The amplified fragment comprises 1655bp，which contains partial RNA-dependent RNA polymerase（RdRp）gene，complete coat protein（CP）gene and complete movement protein（MP）gene. The intergenic region of RdRp gene and CP gene consists 201 nucleotides，which are identical in arrangement to the genus Polerovirus. Molecular homology trees were constructed based on the amino acid sequences of the RdRp，CP and MP，and the results showed that TVDV shares much more homologous with the specieses of the genus Polerovirus than other genuses of family Luteoviridae，which indicated that TVDV should be a definitive member of the genus Polerovirus in the family Luteoviridae.