| CrylAb敏感和抗性亚洲玉米螟氨肽酶N基因的克隆及序列差异分析 |
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| 引用本文： | 徐丽娜,常雪艳,何康来,王振营,白树雄.CrylAb敏感和抗性亚洲玉米螟氨肽酶N基因的克隆及序列差异分析[J].农业生物技术学报,2011,19(1). |
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| 作者姓名： | 徐丽娜 常雪艳 何康来 王振营 白树雄 |
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| 作者单位： | 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京,100193 |
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| 基金项目： | 国家重点基础研究发展规划(973)(No.2007CB109204); 国家自然科学基金(No.30610236)资助 |
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| 摘 要： | 研究亚洲玉米螟(Ostrinia furnacalis)对Cry1Ab产生抗性的分子机理,对今后制定和实施合理的抗性治理策略具有重要的意义。本研究通过PCR方法克隆和测序,鉴定了Cry1Ab敏感-抗性亚洲玉米螟幼虫中肠氨肽酶N(APN)基因家族的一个成员Ofapn3,其cDNA全序列长3591bp,开放阅读框包括3045bp,编码1014个氨基酸。预测蛋白质分子量为115.1kD,等电点(pI)为4.44。其推导的氨基酸序列中具有鳞翅目昆虫氨肽酶典型结构特征,即N-末端具有18个氨基酸的剪切信号肽,谷氨酸锌化氨肽酶保守结构GAMEN,锌结合位点HEXXHX18E,C-末端具有22个氨基酸的糖基磷脂酰肌醇(GPI)锚信号肽。系统分类归为第3支系。与欧洲玉米螟(Ostrinia nubilalis)的Onapn3(GenBank登录号:ABL01483)同源性为96.6%。与Cry1Ab敏感亚洲玉米螟cDNA相比,抗性品系的开放阅读框中有40个碱基发生了点突变,导致氨基酸序列中有10个氨基酸改变。其中Ser735在抗性品系中突变为Pro的现象在抗性棉铃虫APN3的氨基酸突变中也被检测到。鉴定的Cry1Ab敏感和抗...
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| 关 键 词： | 亚洲玉米螟 氨肽酶N Cry1Ab |
Cloning and Sequencing of Aminopeptidase N Genes from Asian Corn Borer Susceptible and Resistant to CrylAb Toxin |
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| Xu Lina,Chang Xueyan,He Kanglai,Wang Zhenying,Bai Shuxiong.Cloning and Sequencing of Aminopeptidase N Genes from Asian Corn Borer Susceptible and Resistant to CrylAb Toxin[J].Journal of Agricultural Biotechnology,2011,19(1). |
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| Authors: | Xu Lina Chang Xueyan He Kanglai Wang Zhenying Bai Shuxiong |
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| Institution: | Xu Lina Chang Xueyan He Kanglai Wang Zhenying Bai Shuxiong State Key Laboratory for Biology of Plant Diseases and Insect Pest,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China |
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| Abstract: | It is meaningful for resistance management to study the resistance mechanism of Asian corn borer (Ostrinia furnacalis) to Cry1Ab. cDNA of aminopeptidase N (APN) from Cry1Ab toxin susceptible and resistant strains of Asian corn borer was cloned and sequenced. Ofapn3,a member of aminopeptidase N families,was identified. It contained 3 591 nucleotides with a 3 045 bp open reading frame that encoded 1 014 amino acids. Putative protein sequences included 8 and 4 potential N-and O-linked glycosylation sites and a... |
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| Keywords: | Asian corn borer Aminopeptidase N Cry1Ab |
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