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| 利用RNAi技术提高玉米直链淀粉含量 | |
| 引用本文： | 郭新梅,张晓东,梁荣奇,陈绪清,杨凤萍,柳青,宋希云.利用RNAi技术提高玉米直链淀粉含量[J].农业生物技术学报,2008,16(4). |
| 作者姓名： | 郭新梅 张晓东 梁荣奇 陈绪清 杨凤萍 柳青 宋希云 |
| 作者单位： | 1. 青岛农业大学生命科学学院,青岛,266109;北京市农林科学院农业生物技术研究中心,北京,100097 2. 北京市农林科学院农业生物技术研究中心,北京,100097 3. 澳大利亚科学与工业研究院,坎培拉,2601 4. 青岛农业大学生命科学学院,青岛,266109 |
| 基金项目： | 国家自然科学基金，北京市自然科学基金 |
| 摘 要： | 为了提高玉米直链淀粉含量，用基因枪将前期工作中构建的sbeⅡb RNAi表达载体pBAC413导入玉米自交系幼胚愈伤组织，经过筛选获得了12 株转化再生植株，其中6 个植株获得了结实种子。DNA点杂交、PCR扩增和PCR－Southern均证明目的基因已整合到T0代再生植株玉米基因组中。对T1代转基因玉米籽粒淀粉含量进行测定，结果表明总淀粉含量比对照没有显著变化，其中1个转基因玉米株系的直链淀粉含量比对照提高了15.6%。 |
| 关 键 词： | 玉米 淀粉分支酶 RNAi 直链淀粉 |
| 收稿时间： | 2007-11-6 |
| 修稿时间： | 2008-4-21 |
of Maize Amylose Content through RNA Interference |
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| GUO Xin-mei,ZHANG Xiao-dong,LIANG Rong-qi,CHEN Xu-qing,YANG Feng-ping,LIU Qing,SONG Xi-yun.of Maize Amylose Content through RNA Interference[J].Journal of Agricultural Biotechnology,2008,16(4). | |
| Authors: | GUO Xin-mei ZHANG Xiao-dong LIANG Rong-qi CHEN Xu-qing YANG Feng-ping LIU Qing SONG Xi-yun |
| Abstract: | To regulate the biosynthesis of maize amylose, an expression vector of RNAi with the fragment of sense gene +FAD2 intron +antisense gene had been constructed, and. was transformed to maize calli which were induced to form from the elite inbred lines by Biolistic PDS1000/He. The selection was carried out on the media containing concentrations of xylose. 12 transformed plantlets were obtained. The integration of alien gene was conformed in 6 transformed plantlets with the analysis of DNA dot blotting, PCR and PCR-Southern hybridization. The seeds amylose content of regeneration plants was detected，and amylose content of a transgenic line increased up to 15.6%. |
| Keywords: | RNAi |
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