两个黄鸡品种Ghrelin基因的PCR-SSCP分析

摘要：本研究针对Genbank上检索的Ghrelin基因的序列设计了两对引物，用PCR-SSCP方法检测了140只京海黄鸡和30只苏禽黄鸡该基因的多态性，结果表明：引物1在两个鸡品种中均未检测到多态；引物2检测到多态：其中京海黄鸡有BB，BC，CC三种基因型，在苏禽黄鸡中只检测到BB，BC两种基因型。在两个鸡品种中B等位基因为优势基因，分布较高，其中在苏禽黄鸡中BB基因型频率达0.933，而京海黄鸡为0.657。对两个纯合基因型测序分析表明：位于Ghrelin基因546bp处发生了T→A的单碱基突变。

PCR-SSCP Analysis on Ghrelin Gene in Yellow Chicken of Two Breeds

Abstract： Two primers are designed on the base of Ghrelin gene sequence in genbank in this study, and the polymorphism of Ghrelin gene in 140 Jinghai yellow chickens and 30 Suqin yellow chickens was analyzed by PCR-SSCP. The result indicates that there were no polymorphism detected by Primer 1,and there were polymorphisms detected by Primer 2. BB, BC and CC genotypes were detected in Jinghai yellow chicken ,but only BB and BC genotypes were detected in Suqin yellow chicken; the allele B was obviously higher than that of C allele in two chicken breeds. The frequency of BB genotype is 0.933 in Suqin yellow chicken and 0.657 in Jinghai yellow chicken. The sequencing results showed that there was one single nucleotide mutation : T→A at the point of 546bp of Ghrelin gene in chicken.