| 棕色棉类黄酮3'-羟化酶基因(F3'H)的克隆及色素合成途径中相关基因表达特性研究 |
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| 引用本文： | 梁明炜,刘海峰,陆雪莹,李艳红,宋武,鲁春芳,肖向文,李晓波.棕色棉类黄酮3'-羟化酶基因(F3'H)的克隆及色素合成途径中相关基因表达特性研究[J].农业生物技术学报,2011,19(5). |
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| 作者姓名： | 梁明炜 刘海峰 陆雪莹 李艳红 宋武 鲁春芳 肖向文 李晓波 |
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| 作者单位： | 1. 中国彩棉(集团)股份有限公司,乌鲁木齐,830016
2. 中国科学院新疆理化技术研究所,新疆植物资源化学重点实验室,乌鲁木齐830011 |
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| 基金项目： | 中国科学院新疆理化技术研究所博士启动资金项目、中国科学院“西部之光”人才培养计划项目(No.RCPY200802); 转基因生物新品种培育重大专项项目(No.2009ZX08005-027B)共同资助 |
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| 摘 要： | 为探讨花色苷途径在彩棉色素形成中的作用及彩棉色素形成规律,本研究根据葡萄(Vitis vinifera)的类黄酮3'-羟化酶(flavonoid3'-hydroxylase,F3'H)基因全长cDNA序列blast所得棉花(Gossypium hirsutum)的EST序列(GenBank登录号:DT545210,CO071403和BG447485)设计引物,以开花后16d的新彩棉5号(XC-5)纤维为材料,采用RACE(rapid amplification of cDNA ends)和RT-PCR方法分离得到了2个类黄酮3'-羟化酶基因的全长cDNA序列,长度为1761和1892bp,均含有一个97~1629bp、长度为1533bp的开放阅读框,编码510个氨基酸,将这两个序列命名为GhF3'H-1和GhF3'H-2,分别提交GenBank,登录号为HM598123和HM598124,此2个序列编码区完全相同,仅在3'非翻译区(UTR)存在片段长短的差异。半定量RT-PCR检测花色苷合成途径中查尔酮合成酶(chalcone synathase,CHS)基因、F3'H、类黄酮3',5'-羟化酶(flavon...
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| 关 键 词： | 类黄酮3'-羟化酶 棕色棉 RACE |
Cloning of Flavonoid 3'-hydroxylase Gene (GhF3'H) and Expressional Characteristics of Several Genes Associated with Pigment Synthesis in Brown Cotton (Gossypium hirsutum L.) Fibers |
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| Liang Mingwei,Liu Haifeng,Lu Xueying,Li Yanhong,Song Wu,Lu Chunfang,Xiao Xiangwen,Li Xiaobo.Cloning of Flavonoid 3'-hydroxylase Gene (GhF3'H) and Expressional Characteristics of Several Genes Associated with Pigment Synthesis in Brown Cotton (Gossypium hirsutum L.) Fibers[J].Journal of Agricultural Biotechnology,2011,19(5). |
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| Authors: | Liang Mingwei Liu Haifeng Lu Xueying Li Yanhong Song Wu Lu Chunfang Xiao Xiangwen Li Xiaobo |
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| Institution: | Liang Mingwei Liu Haifeng2 Lu Xueying1 Li Yanhong1 Song Wu2 Lu Chunfang1 Xiao Xiangwen1 Li Xiaobo1 1 Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Xinjiang Key Laboratory of Plant Resource and Natural Products Chemistry,Urumqi 830011,China,2 China Colored-cotton (Group) Co.,Ltd.,Urumqi 830016 |
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| Abstract: | The purpose of this research is to explore the role of anthocyanidin synthesis pathway in pigmentation of brown cotton fibers. Two full-length cDNAs encoding flavonoid 3' -hydroxylase gene (F3'H) was cloned from XC-5 fibers of 16 days post anthesis (DPA) by rapid amplification of cDNA ends (RACE) and RT-PCR using specific primers based on the Gossypium hirsutum EST sequences(GenBank accession: DT545210, CO071403 and BG447485)from the blast results with the full-length cDNA of F3'H in Vitis vinifera that had... |
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| Keywords: | Flavonoid 3'-hydroxylase Brown fiber cotton Rapid amplification of cDNA ends (RACE) |
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