| 松材线虫及其近缘种热激蛋白90基因(hsp90)克隆与序列分析 |
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| 引用本文： | 代玲玲,彭德良,黄文坤,彭焕,龙海波,王高峰.松材线虫及其近缘种热激蛋白90基因(hsp90)克隆与序列分析[J].农业生物技术学报,2011,19(5). |
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| 作者姓名： | 代玲玲 彭德良 黄文坤 彭焕 龙海波 王高峰 |
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| 作者单位： | 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193 |
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| 基金项目： | 国家重点基础研究发展规划(973)项目(No.2009CB119200)资助 |
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| 摘 要： | 热激蛋白90家族(heat shock protein 90(HSP)family)是一种进化保守的蛋白质,在分子伴侣功能、细胞循环调控、抗原传递方面发挥着重要作用。本研究用RT-PCR和RACE技术,克隆得到了松材线虫(Bursaphelenchus xylophilus)、拟松材线虫(Bursaphelenchus mucronatus)和豆伞滑刃线虫(Bursaphelenchus doui)热激蛋白90基因的cDNA全长序列,分别命名为Bx-hsp90、Bm-hsp90和Bd-hsp90,(GenBank登录号分别为:GU013561、HMO34943和HM347331),其cDNA全长分别为2255、2193和2232bp,开放阅读框均为2127bp,编码708个氨基酸,5'端非编码区的长度分别为33、13和13bp,3'端非编码区的长度分别为95、53和92bp。其DNA全长分别为2440、2388和2407bp,包含3个内含子。三者氨基酸序列的一致性为98.78%。进化分析表明,松材线虫、拟松材线虫、豆伞滑刃线虫与秀丽小杆线虫(Caenorhabditis elegans)的亲缘关系较近,推测三...
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| 关 键 词： | 松材线虫 拟松材线虫 豆伞滑刃线虫 热激蛋白90 基因克隆 |
Cloning and Sequence Analysis of Three New Heat Shock Protein 90 Genes(hsp90) from Bursaphelenchus xylophilus, B. mucronatus and B. doui |
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| Dai Lingling,Peng Deliang,Huang Wenkun,Peng Huan,Long Haibo,Wang Gaofeng.Cloning and Sequence Analysis of Three New Heat Shock Protein 90 Genes(hsp90) from Bursaphelenchus xylophilus, B. mucronatus and B. doui[J].Journal of Agricultural Biotechnology,2011,19(5). |
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| Authors: | Dai Lingling Peng Deliang Huang Wenkun Peng Huan Long Haibo Wang Gaofeng |
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| Institution: | Dai Lingling Peng Deliang* Huang Wenkun Peng Huan Long Haibo Wang Gaofeng State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Science,Beijing 100193,China |
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| Abstract: | Heat shock protein 90(HSP90) family is an evolutionarily conserved protein, to be involved in chaperone function, cell cycle control and antigen presentation. The cDNA sequences of heat shock protein 90 from Bursaphelenchus xylophilus, B. mucronatus and B. doui were cloned by RT-PCR and RACE methods, and named Bx-hsp90, Bm-hsp90, Bd-hsp90 (GenBank Accession No. GU013561, HMO34943 and HM347331) respectively. Theire full cDNA length were 2 255, 2 193 and 2 232 bp, the ORF were all 2 127 bp encoding 708 amino ... |
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| Keywords: | Bursaphelenchus xylophilus Bursaphelenchus mucronatus Bursaphelenchus doui Heat shock protein 90 Gene clone |
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