首页 | 本学科首页   官方微博 | 高级检索  
     检索      

利用微卫星标记分析6个山羊品种遗传多样性
引用本文:张爱玲,马月辉,李宏滨,关伟军,浦亚斌,张英汉,陈宏,赵倩君,何晓红.利用微卫星标记分析6个山羊品种遗传多样性[J].农业生物技术学报,2006,14(1):38-44.
作者姓名:张爱玲  马月辉  李宏滨  关伟军  浦亚斌  张英汉  陈宏  赵倩君  何晓红
作者单位:1. 中国农业科学院畜牧研究所,北京,100094;西北农林科技大学动物科技学院,杨凌,712100
2. 中国农业科学院畜牧研究所,北京,100094
3. 西北农林科技大学动物科技学院,杨凌,712100
基金项目:中国科学院资助项目;科技部基础性工作项目;科技部专项基金
摘    要:利用微卫星标记技术,分析了山西黎城大青羊、吕梁黑山羊、阳城白山羊、新疆南疆山羊、新疆北疆山羊以及宁夏中卫山羊6个地方山羊品种的分子水平上的遗传多样性,结果表明:19个微卫星位点均为中度和高度多态位点,平均多态信息含量(PIC)达0.6859"0.7083;6个品种平均位点杂合度在0.3832"0.4277之间。遗传分化系数表明:BM203、BM023、BMS6526和BM8754个位点的遗传分化系数(GST)估算值较大,分别为0.0475、0.0491、0.0107和0.0262,BM3033值最小,为0,表明前4个位点在不同品种间的基因分化占总群杂合度的4.75#、4.91#、1.07#和2.6#;其它位点遗传分化系数(GST)值不高,遗传变异主要存在于各个品种内,品种间遗传变异不大。以Nei氏标准遗传距离的UPGMA和N-J聚类结果表明,吕梁黑山羊与阳城白山羊具有较近的亲缘关系,黎城大青羊与新疆南疆山羊具有较近的亲缘关系;以共祖距离的UPGMA和N-J聚类结果,阳城白山羊与新疆南疆山羊亲缘关系较近,山西地方山羊与新疆山羊有可能存在一定的基因交流。

关 键 词:山羊  微卫星  遗传多样性  系统发生  遗传分化系数
文章编号:1006-1304(2006)01-0038-07
收稿时间:2005-01-05
修稿时间:2005-02-09

Genetic Diversity of Six Chinese Indigenous Goat Breeds Using Microsatellite Analysis
ZHANG Ai-Ling,MA Yue-Hui,LI Hong-Bin,GUAN Wei-jun,PU Ya-Bin,ZHANG Ying-Han,CHEN Hong,ZHAO Qian-Jun,HE Xiao-Hong.Genetic Diversity of Six Chinese Indigenous Goat Breeds Using Microsatellite Analysis[J].Journal of Agricultural Biotechnology,2006,14(1):38-44.
Authors:ZHANG Ai-Ling  MA Yue-Hui  LI Hong-Bin  GUAN Wei-jun  PU Ya-Bin  ZHANG Ying-Han  CHEN Hong  ZHAO Qian-Jun  HE Xiao-Hong
Institution:1.Insfitute of Animal Science, China Academy of Aricultural Science, Beijing 100094, China; 2.College of Animal Science and Technology, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Abstract:The genetic diversity of six native goats in China was surveyed using 19 microsatellite loci. The six goats included Licheng blue goat, lvliang black goat, Yangcheng white goat, Xinjiang goat in North, Xinjiang goat in Sourth and Zhongwei goat in Ningxia. The 19 microsatellite loci showed middle high and high polymorphism information content value. The mean polymorphism information content value was between 0.6859-0.7083, which indicated that the 6 breeds had abundant genetic diversity. The mean heterozygosity was between 0.3832-0.4277; the coefficient ofgene differentiation was large in the BM203, BM023, BMS6526, BM875 loci, which were 0.0475,0.0491,0.0107 and 0.0262.respectively; the one of BM3033 was 0. The four allelic gcne differentiation are 4.75%,4.91%, 1.07% and 2.6% respectively, of total gcne diversity respectively. It showed that the gcne differentiation was remarkable in every breeds but not between the breeds. In the UPGMA dendrogram and N-J based on the Nei genetic distance, Lvliang black goat and the Yangcheng white goat had the intimate relationship; Lichcng goat and Xinjiang nanjiang goat had the close relationship;In the UPGMA dendrogram and NJ dcndrogram by coancestry distance,Yangcheng goat had the close relationship with Xinjiang nanjiang goat.lt suggested there were gcne exchange between Xinjiang goat and the Shanxi goat.
Keywords:goat  microsatellite  genetic diversity  genetic distance  dendrograms  Coefficient of gene differentiation
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号