| 花药特异嵌合启动子的构建及雄性不育转基因拟南芥的获得 |
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| 引用本文： | 陈潜,汪迎春,张利明,李文彬,孙勇如.花药特异嵌合启动子的构建及雄性不育转基因拟南芥的获得[J].农业生物技术学报,2001,9(1):62-64. |
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| 作者姓名： | 陈潜 汪迎春 张利明 李文彬 孙勇如 |
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| 作者单位： | 中国科学院遗传学研究所, |
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| 基金项目： | 863计划资助项目（863-101-01-04-02） |
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| 摘 要： | 为了克服TA29／Barnase转基因雄性不育植物的温度敏感性，在TA29启动子上游串联了CaMV35 S启动子，同时在CaMV35 S启动子上游串联一个调节序列antherbox，构建成嵌合启动子antherbox-CaMV35 S-pTA29。该启动元件调控的GUS基因瞬间表达实验表明它是花药颈毡层特异的启动子。这个启动元件及其调控下的barnase基因构建植物表达载体转化，拟南芥菜，获得了雄性不育的转基因植株。
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| 关 键 词： | 嵌合启动子 雄性不育 拟南芥菜 转基因植物 花药 |
| 修稿时间： | 2000年5月29日 |
Construction of Anther Specific Chimeric Promoter and Further Establishment of Transgenic Artificial Male Sterile Arabidopsis thaliana |
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| Chen Qian,Wang Yingchun,Zhang Liming,Li Wenbin,Sun Yongru.Construction of Anther Specific Chimeric Promoter and Further Establishment of Transgenic Artificial Male Sterile Arabidopsis thaliana[J].Journal of Agricultural Biotechnology,2001,9(1):62-64. |
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| Authors: | Chen Qian Wang Yingchun Zhang Liming Li Wenbin Sun Yongru |
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| Abstract: | The artificial male sterility generated by introducing TA29/Barnase into target plants usually shows temperature sensitiveness.In order to overcome this significant shortcoming, here we added a CaMV35 S promoter together with a regulatory segment-antherbox into the upstream of TA29,then came cameric promoter antherbox-CaMV35S-TA29.Using the GUS transient expression assay,we further proved that the antherbox-CaMV35S-pTA29 regulates GUS expression in an anther tapetum specific manner.Then,by transforming Arabidopsis thaliana with antherbox-CaMV35S-pTA29 driven barnase gene, we successfully get the trangenic artifici
al male sterile lines. |
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| Keywords: | chimeric promoter male sterility Arabidopsis thaliana transgenic plant |
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