利用SSR和RAPD标记分析中国部分地区晚疫病菌群体遗传多样性

利用SSR和RAPD对来自福建、黑龙江、河北和内蒙古4个地理群体的80个马铃薯晚疫病菌（phytophthora infestans）株进行了遗传多样性分析。13对SSR引物共扩增出76条谱带，多态性条带比率78.9%，相似系数变化范围0.00～0.42之间；筛选出的14条RAPD引物共扩增出189条谱带，多态性条带比率95.2%，相似系数变化范围0.04～0.66之间。遗传多样性分析表明，在4个群体中，福建群体的多样性更为丰富。遗传相似性分析显示，黑龙江和内蒙古两个群体间的遗传相似性最高，而福建和河北两个群体间的遗传相似性最低。聚类分析显示，来自南方福建的菌株与来自北方黑龙江、河北和内蒙古的菌株亲缘关系较远，且福建群体分布于更多的聚类组，显示出更高的遗传变异度。

Population Genetic Diversity of Phytophthora infestans from China Revealed by SSRs and RAPDs

SSR and RAPD molecular markers were used to assess the genetic diversity among 80 isolates of Phytophthora infestans on potato(Solanum tuberosum) from Fujian, Heilongjiang, Hebei and Inner-mongolia. Polymorphism was identified by 13 SSR primers and 14 RAPD primers in the isolates of P. infestans on potato. A total of 76 bands were produced by SSRs with polymorphic bands accounting for 78.9% and similarity coefficient within 0.00 to 0.42. And a total of 189 bands were generated by RAPDs with polymorphic bands accounting for 95.2% and similarity coefficient ranging from 0.04 to 0.66. Analysis of genetic variation showed that there existed higher genetic variation in the Fujian populations in comparison to the populations of Heilongjiang, Hebei and Inner-mongolia. Nei's genetic identity analysis indicated that the populations of Heilongjiang and Inner-mongolia were closer than Fujian and Hebei populations revealed by SSRs and RAPDs. The results of cluster analysis revealed that some isolates from Fujian, the south region of China were distantly related to some isolates from Heilongjiang, Heibei and Inner-mongolia, the north of China and the Fujian populations had a higher genetic diversity than that of the others.