山羊美臀突变基因（CLPG）的多态性鉴定及其与生产性能的关系

Callipyge绵羊的臀部肌肉明显肥大，呈极性超显性遗传。研究克隆了山羊（Capra hircas）美臀突变基因(CLPG) 250 bp的序列(GenBank登录号：EU753362), 对其多态性及其与山羊生产性能的关系进行了分析。结果表明，所扩增片段与绵羊和猪相应片段的同源性分别为96.04%和88.65%。PCR-SSCP分析表明，在该DNA区段存在多态性。测序结果显示，在对应于绵羊（Ovis aries）的CLPG基因没有发现A→G的突变，但在该位点下游147 bp处检测到一个A→C的颠换，记为SNP216。对波尔山羊(n　=63)、莱芜黑山羊(n　=70)、鲁波杂交山羊(n　=40)、鲁北白山羊(n　=29)和内蒙古阿拉善白绒山羊(n　=115)共5个群体的多态性进行了检测，结果表明：除鲁北白山羊以外，A等位基因均为优势等位基因；波尔山羊、莱芜黑山羊、鲁北白山羊和鲁波杂交山羊群体均处于Hardy-Weinberg平衡状态（P　＞0.05），而内蒙古阿拉善白绒山羊群体显著偏离Hardy-Weinberg平衡状态（P　＜0.01）；不同基因型的内蒙古阿拉善白绒山羊的初生重、从出生到断奶时的增重及产绒量的最小二乘均值差异不显著（P　＞0.5）。

Identification of Polymorphism of the Callipyge Mutation Gene (CLPG) in Goats and Its Associations with Production Traits

The Dorset ram with Callipyge phenotype shows muscular hypertrophy at buttock, and its inheritance is polar overdominance. A partial DNA fragment of 250 bp in goat (Capra hircas) Callipyge mutation gene(CLPG) (GenBank Accession No.EU753362) was obtained, and had a 96.04% and 88.65% identity with the corresponding region of ovine(Ovis aries) and porcine CLPG, respectively. And a polymorphism in the DNA fragment was detected by PCR-SSCP. Sequencing results indicated that no A→C mutation was corresponding to ovine CLPG, whereas a A→C transversion located 147 bp downstream from the CLPG site was identified. The polymorphism was named after its position as SNP216 was investigated in Boer (n = 63), Laiwu Black (n = 70), Lubei White × Boer Hybrid (n = 40), Lubei White (n = 29) and Inner Mongolia Alashan white cashmere (n = 115) goat populations. The results indicated that allele A was predominant in the four goat populations of Boer, Laiwu Black, Lubei White × Boer Hybrid and Inner Mongolia Alashan white cashmere, and except for the Inner Mongolia Alashan White cashmere goat, the other four populations were in the state of Hardy-Weinberg equilibrium (P > 0.05). In Inner Mongolia Alashan white cashmere goats, least square means of birth weight, production of cashmere and body weight gain from birth to weaning between AA and AC were not significantly different (P >0.5).