商陆抗病毒蛋白基因转化芥菜的初步研究

以芥菜(Brassica Juncea Coss.)的子叶为外植体，通过根癌农杆菌（Agrobacterium tumefaciens）的介导，将商陆抗病毒蛋白（PAP）基因导入到芥菜中。对所获得的40株抗性植株进行PCR扩增，其中阳性植株为29株；Southern blot分析结果证明PAP基因已被整合到芥菜基因组中，在大多数转基因植株中外源基因呈单挎贝，少数为双挎贝；Northern blot分析结果表明，PAP在转基因植株中正常表达。转基因植株接种病毒试验初步结果表明，转PAP基因的植株对TuMV的侵染有一定的抑制作用。

The study of Transformation of Mustard (Brassica Juncea Coss.) with Pokeweed Antiviral Protein Gene

The pokeweed antiviral protein (PAP) gene was transferred into the cotyledons of mustard (Brassica Juncea Coss.) mediated by Agrobacterium tumefaciens. Using PCR technology to analyse 40 anti-kanamycin plants, 29 positive plants were obtained. The result of Southern blot analysis was that there were single copy or double copies of transgene in the transgenic plants. The result of Northern blot analysis was that the PAP gene was normally expressed in transgenic plants. The results of transgenic plants inoculated with virus TuMV showed that the transgenic plants were more resistant to TuMV than non-transgenic plants.