对生玉米互生玉米内源激素含量的差异分析

摘要：利用植物激素ELISA方法测定和分析不同叶序玉米的不同组织、不同生育期内源细胞分裂素（ZRs、DHZRs、iPAs）、内源生长素（IAA）含量差异，结果表明：不同叶序玉米在不同生育期幼胚、成熟花粉、成熟叶片内源ZRs、DHZRs、iPAs和IAA均无显著差异；不同生育期对生叶序玉米幼苗顶端分生组织、成熟苗顶端雄幼穗ZRs、DHZRs、iPAs含量显著高于的互生玉米，IAA含量在两者之间无显著差异；对生叶序玉米幼苗顶端分生组织、成熟苗顶端雄幼穗CTK/IAA比值显著高于同期近等基因系互生叶序玉米.但在不同生育期幼胚、叶片及花粉内均无显著差异。对生叶序的形成与生长点组织细胞分裂素含量增加密切相关,玉米生长点CTK/IAA域值的稳定和变化是调节叶序稳定和变异重要因素。三类内源细细胞分裂素同步增加预示该对生叶序突变的分子基础可能是细胞分裂素合成基因上游调控基因的变异所致。

Studies on Endogenous Hormones Content Comparison Between Decussate Maizes and Alternative Maizes

Abstract: This study was aimed to compare the contents of endogenous cytokine (ZRs、DHZRs、iPAs ) and auxin (IAA) in decussate and alternative maize by the ELISA method .Two sets of near isogenic lines of 8701d, 8701D and H4d, H4D were used in these examinations. In this study, the results showed that the maize with different phyllotary leaf had same level of endogenous cytokine (ZRs、DHZRs、iPAs ) and auxin in mature pollen ,different development leaf and young embryo. However，the contents of above cytokine in immature male spikes from decussate plants and SAMs were great higher than that in isogenic alternative maize. At the same time, there was no significant difference in the level of IAA among them. Further analysis indicated that the value of CTK/IAA in decussate maize was not only dominant higher than in alternative maize, but also was increased accompanied with phyllotary changes from alternative to decussate. Taken together, we can draw a conclusion that there is close relativity between the decussate phenotype and the level of SAM cytokines. The stabilization and change of CTK/IAA is an important factor which regulates phyllotary development.The producing of decussate mutant is relative to the change of endogenous hormone content in growthing tissue.