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Mn2+对白腐菌发酵油菜秸秆木质纤维素酶活性及组织结构的影响
引用本文:王力生,程建波,朱洪龙,蔡海莹,方飞,程茂基.Mn2+对白腐菌发酵油菜秸秆木质纤维素酶活性及组织结构的影响[J].农业生物技术学报,2012,20(6):663-668.
作者姓名:王力生  程建波  朱洪龙  蔡海莹  方飞  程茂基
作者单位:1. 安徽农业大学动物科技学院,合肥,230036
2. 南京农业大学动物科技学院,南京,210095
基金项目:长三角联合攻关项目,科技人员服务企业行动项目,安徽省肉牛产业技术体系专项经费项目
摘    要:白腐菌对木质素有很强的降解能力。本研究选择白腐菌(Pleurotus ostreatu)WY01于限氮培养基添加不同水平Mn2+固态发酵油菜(Brassica campestris L.)秸秆,实验分为对照组(SMc)、实验1组(SM1)和实验2组(SM2)(Mn2+浓度分别为0、0.6和1.2mmol/L),于28℃恒温培养,静止发酵40d。结果表明,漆酶(Lacs)和锰依过氧化物酶(MnPs)活性SM1极显著高于其它两组(P<0.01),峰值分别在第6天211.11U/g和第10天49U/g;Mn2+对木质素过氧化物酶(Lips)、纤维素酶(Cels)和半纤维素酶(Hcels)活性没有影响,且秸秆组织结构变化与相关纤维素酶活性具有一致性。电镜扫描显示,未经任何处理的油菜秸秆组织结构十分致密,细胞组织排列有序,经白腐菌WY01处理,第20天SMc油菜秸秆原来致密有序的组织遭到破坏,部分出现疏松的网状,SM1第20天油菜秸秆组织遭到破坏且局部出现坍塌现象,第40天纤维组织完全分解并粉化;SM2第20天秸秆组织也遭到不同程度的破坏,第40天纤维组织完全分解,但效果不及SM1。综合生物降解效果SM1>SM2>SMc。结果提示:Mn2+能够诱导并有利于白腐菌WY01对油菜秸秆的降解。本研究为油菜秸秆开发生物饲料提供依据。

关 键 词:糙皮侧耳  Mn2+  油菜秸秆  固体培养  酶活  组织结构

Effect of Different Mn2+ Levels on Activity of Lignocellulolytic Enzymes and Tissue Structure in Rape Straw Fermented by Pleurotus ostreatus
WANG Li-Sheng , CHENG Jian-Bo , ZHU Hong-Long , CAI Hai-Ying , FANG Fei , CHENG Mao-Ji.Effect of Different Mn2+ Levels on Activity of Lignocellulolytic Enzymes and Tissue Structure in Rape Straw Fermented by Pleurotus ostreatus[J].Journal of Agricultural Biotechnology,2012,20(6):663-668.
Authors:WANG Li-Sheng  CHENG Jian-Bo  ZHU Hong-Long  CAI Hai-Ying  FANG Fei  CHENG Mao-Ji
Institution:1 College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China;2 College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
Abstract:White-rot fungus(Pleurotus ostreatu) has strong degradation ability of lignin.The experiment was conducted to research effect of different Mn2+ levels on lignocellulose enzyme activity of rape(Brassica campestris L.) straw fermented by white-rot fungus and changes of rape straw tissue structure.The experiment,which was separated into control group(SMc,0 mmol/L),Test One(SM1,0.6 mmol/L) and Test Two(SM2,1.2 mmol/L),selected white-rot fungi P.ostreatu-WY01 to ferment rape straw motionlessly fermented of 40 d on constant temperature of 28℃ in nitrogen-limited mediums which were added Mn2+ of different levels in solid state.The result showed that laccase activity and manganese peroxidase(MnPs) activity of Test One,which reached respectively peak of 211.11 U/g at 6 day and 49 U/g at 10 day,very significantly overtoped other groups(P<0.01);lignin peroxidase(Lips),cellulose enzyme(Cels) and hemicellulase enzyme(Hcels) activity respectively were no difference by adding Mn2+ and tissue structure of straw was accordance with related cellulose enzyme activity.At early period of fermentation,experimental groups Lacs activity reached peak at 6 d and fell fastly until tend to smooth at 10 d while SMc had been living in lower level in process.Lips of SM1 reached peak at 12 d while SM2 and SMc reached peak at 10 d.After peaks,they trended to fall gradually until keep lower level after 20 d.MnPs activity kept low level before 6d of fermentation and ascended suddenly followly.Experimental groups MnPs activity which SM1 and SM2 respectively reached peak at 10 d and 16 d,were greater significantly than SMc.During the fermentation,all treatment groups Cels and Hcels activity had a rising trend and experimental groups were higher than control group.Scanning electron microscopy showed that without any treatment,tissue was very compact and tissue arranged orderly.Empty cavity the arrow refered to the figure might be caused by high temperature sterilization leading to inflation.Processed by the white-rot fungus P.ostreatus-WY01,SMc rape straw tight tissue was destroyed and appeared some loose mesh at 20 d;straw waxy skin arrowed to on left and fibrous tissue was broken down on the right at 40 d;SM1 partly appeared collapsed at 20 d and fibrous tissue was completly decomposed and pulverized at 40 d.SM2 tissue was destroyed in different degree at 20 d and fibrous tissue was completed decomposed at 40 d while it not as effective as SM1.The findings suggested that Mn2+ could induce and benefit degradation of rape straw by white-rot fungus(P.ostreatu-WY01),and comprehensive biodegradation effect was SM1>SM2>SMc.The study provides us a basis to develop resources of biological feed using rape straw.
Keywords:Pleurotus ostreatus  Mn2+  Rape straw  Solid culture  Enzyme activity  Tissue structure
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