| 苏云金芽胞杆菌Bt9875杀虫晶体蛋白可诱导Caspase和Bcl-2家族参与调控HL-60细胞凋亡 |
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| 引用本文： | 竺利红,施跃峰,吴吉安,梁建根,李翀.苏云金芽胞杆菌Bt9875杀虫晶体蛋白可诱导Caspase和Bcl-2家族参与调控HL-60细胞凋亡[J].农业生物技术学报,2010,18(1):92-97. |
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| 作者姓名： | 竺利红 施跃峰 吴吉安 梁建根 李翀 |
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| 作者单位： | 1. 浙江省农业科学院植物保护与微生物研究所,杭州,310021
2. 北京大学医学部基础医学院.北京.100083 |
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| 摘 要： | 研究Caspase家族与Bcl-2家族参与调控苏云金芽胞杆菌(Bacillus thuringiensis,Bt)Bt9875杀虫晶体蛋白对人急性髓细胞性白血病细胞HL-60的影响.本实验采用MTT法检测了杀虫晶体蛋白诱导HL-60细胞凋亡后的Caspase家族的活性和Caspase凋亡酶抑制剂对杀虫晶体蛋白诱导HL-60细胞凋亡的影响;采用Western blot检测了杀虫晶体蛋白诱导多聚ADP-核糖聚合酶(PARP)降解、Bcl-2/Bax调控和细胞色素C的释放.研究结果表明,杀虫晶体蛋白作用HL-60细胞后,激活了Caspase-3、Caspase-8和Caspase-9,在48 h内Caspase家族抑制剂(Z-VAD-FMK)、Caspase-3抑制剂(z-DEVD-FMK)和Caspase-9抑制剂(Z-LEHD-FMK)均可显著抑制杀虫晶体蛋白诱导的细胞凋亡;杀虫晶体蛋白可明显上调促凋亡蛋白Bax的表达,同时下调抗凋亡蛋白BCl-2的表达,并观察到胞浆中细胞色素C的释放.初步证明了Bt9875杀虫晶体蛋白诱导的HL-60细胞凋亡是由Caspase家族和Bcl-2家族共同调控的,线粒体途径在诱导细胞凋亡过程中起着重要作用.
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| 关 键 词： | 苏云金芽胞杆菌 杀虫晶体蛋白 HL-60细胞 凋亡 |
Both Caspase Family and Bcl-2 Family were Involved in Regulating Apoptosis in HL-60 Cells Induced by Bacillus thuringiensis Bt9875 Insecticidal Crystal Proteins |
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| Zhu Lihong Shi Yuefeng Wu Ji'an Liang Jiangen Li Chong Institute of Plant Protection , Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou, School of Basic Medical Sciences,Peking University,Beijing.Both Caspase Family and Bcl-2 Family were Involved in Regulating Apoptosis in HL-60 Cells Induced by Bacillus thuringiensis Bt9875 Insecticidal Crystal Proteins[J].Journal of Agricultural Biotechnology,2010,18(1):92-97. |
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| Authors: | Zhu Lihong Shi Yuefeng Wu Ji'an Liang Jiangen Li Chong Institute of Plant Protection Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou School of Basic Medical Sciences Peking University Beijing |
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| Institution: | Zhu Lihong 1 Shi Yuefeng 1 Wu Ji'an 1 Liang Jiangen 1 Li Chong 2* 1 Institute of Plant Protection , Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou,310021,2 School of Basic Medical Sciences,Peking University,Beijing,100083 |
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| Abstract: | To study the effect of Caspase and Bcl-2 families on apoptosis HL-60 cells induced by insecticidal crystal proteins from Bacillus thuringiensis Bt9875,the activation of Caspases and the effect of Caspase inhibitors on apoptosis of HL-60 cells were detected.The cleavage of poly ADP-ribose polymerase(PARP),Bcl-2/Bax regulation and the release of cytochrome C were observed by Western blot analysis.Insecticidal crystal proteins from Bacillus thuringiensis Bt9875 can activate Caspase-3,Caspase-8 and Caspase-9.Mo... |
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| Keywords: | Caspase Bacillus thuringiensis Crystal proteins HL-60 cell Caspase Apoptosis |
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