奶牛乳腺炎金黄色葡萄球菌临床分离株生化特性与菌体蛋白免疫原性分析

摘要：病原学调查发现金黄色葡萄球菌是奶牛乳腺炎的最主要致病菌之一。从53株金黄色葡萄球菌乳样分离株中挑选了培养和形态典型的六株进行生化试验和传代稳定性试验，进一步研究这六株菌的凝固酶、溶血素等致病性因子和耐药性。对菌体裂解产物进行蛋白电泳图谱分析发现菌体蛋白条带具有很高的相似性,TQ-1株电泳蛋白条带密度较其它几株高。用其中四株生化典型、传代稳定、致病因子较多的菌株免疫兔，制备高免血清，分析全菌抗原的蛋白转印图谱，发现XG-2株在52KD处有特殊保护性抗原条带。综合本文各试验结果，确定以TQ-1和XG-2株为疫苗候选株。

Analysis of the Biochemical Characters and Antigenicity of the Staphylococcus aureus Isolated from Milk of Dairy Cows Suffering from Mastitis

Abstract: Fifty-three strains of Staphylococcus. aureus were isolated and identified from 93 milk samples of dairy cows suffering from mastitis, it’s the main pathogen in the three cow fields in Nanjing region. From the isolates six typical strains were selected to analysis with the biochemical characters and the inherited stability. Furthermore the other pathogenic factors of the six isolates were detected, such as coagulase, hemolysin and antimicrobial resistance factors etc. The crushed bacterial cells of 6 strains and 1 reference strain were processed with SDS-PAGE. The protein maps of the strains are similar except for the higher protein density of TQ-1. Mixture of whole bacterial cells of four S. aureus isolates(XL-1、TQ-1、XG-2 andT Q-2) were chosen to inoculate rabbit, and the hyperimmunized serum was used in the Western-blot assay to analyze the cross protective antigens of the isolates. The result shows the isolate XG-2 has unique band whose molecular mass is about 52KD in the NC membrane. According to the results, 2 S. aureus isolates were selected as the strains for preparing vaccine against bovine mastitis.