The Tolerance Index and Translocation Factor were Used to Identify the Barley Genotypes with High Arsenic Stress Tolerance

Ninety-four barley genotypes were used to investigate the genotypic differences in arsenic (As) uptake and translocation and their relationships with As tolerance index (TI) and translocation factor (TF). Two As treatments (300 µM and 500 µM) were applied in the initial screening and the confirmatory experiments, respectively. The results showed significant (p < 0.05) differences in tissue biomass, shoot height, root length, As TI and TF among genotypes. Based on As TI, 11 barley genotypes were selected and divided into 3 groups, i.e. tolerant, mildly tolerant and sensitive. There was more As uptake in the roots of the As tolerant genotypes, while the As sensitive genotypes contained more As in shoots, which was further proved by the greater TF. Significantly negative correlation was observed between shoot and root As concentration. The results showed that As tolerant genotypes are able to restrict the upward movement of As, thus developing their tolerance.