施氮量对不同小麦品种高分子量麦谷蛋白亚基表达量及品质影响的研究

采用盆栽试验和SDS-PAGE技术，研究了氮肥对强筋和中筋小麦亚基表达量、品质性状及相关关系的影响。结果表明，施用氮肥提高了优质小麦高分子量谷蛋白亚基表达量，对强筋小麦影响较小，而对中筋小麦影响较大。施氮后，强筋小麦的亚基表达量与醇溶蛋白和谷蛋白的含量极显著相关，而中筋小麦的亚基表达量仅与谷蛋白的含量显著相关，说明氮肥对不同亚基组成小麦高分子量谷蛋白表达量及蛋白组分调控有一定差异。施氮后，高分子量谷蛋白亚基表达量与各项加工品质指标呈正相关；强筋小麦的亚基表达量与湿面筋含量、稳定时间、沉降值和评价值相关性达到显著或极显著水平；而中筋小麦的亚基表达量仅与沉降值相关性达到显著水平。

Effect of nitrogen application on high molecular weight glutenin subunit expression and grain quality of winter wheat

A pot experiment was carried out to study High Molecular Weight Glutenin Subunit (HMW-GS) expression, quality and their relationship in different gluten wheat types at different N-levels, using SDS-PAGE technology. Two wheat cultivars, strong gluten wheat Linyou 145（LY145） and medium gluten wheat Linyou 2018（LY2018）, were used and five rates of N fertilizer were applied (N 0, 0.667, 1.332, 1.998 and 2.664 g/pot, respectively), with randomized block design. The results showed that, nitrogen application increased HMW-GS expression, and the effect on medium gluten wheat was greater than on strong gluten wheat. The effect of nitrogen application on HMW-GS expression with protein composition differed among subunit type of high quality wheat. Correlation coefficients between HMW-GS expression with gliadin and glutenin in strong gluten wheat were both at 1% significance levels and the correlation coefficient with glutenin in medium gluten wheat was at 5% level. HMW-GS expression was positively correlated with processing quality characteristics. HMW–GS expression in strong gluten wheat was significantly correlated with wet gluten content, stability time, sedimentation value and evaluation value, while HMW–GS expression in medium gluten wheat was only significantly correlated with sedimentation value.