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中国十三省市土壤中非共生固氮微生物菌种资源研究
引用本文:孙建光,徐晶,胡海燕,张燕春,刘君,王文博,孙燕华.中国十三省市土壤中非共生固氮微生物菌种资源研究[J].植物营养与肥料学报,2009,15(6):1450-1465.
作者姓名:孙建光  徐晶  胡海燕  张燕春  刘君  王文博  孙燕华
作者单位:1.农业部作物营养与施肥重点实验室 中国农业科学院农业资源与农业区划研究所 北京 100081
基金项目:农业部"948"项目,国家863项日,国家自然科技资源平台项日 
摘    要:从全国13个省市自治区的70份土样中分离、采集到了非共生固氮微生物资源181份。形态特征、生理生化特征和16S rDNA序列分析表明,采集到的菌种资源在科学分类上属于24属66种,大约占到已报道非共生固氮微生物属的一半,具备一定的多样性和代表性。资源在分类学上的特点是分类地位相对集中,有65株菌属于类芽孢杆菌属,占总量的36%;52株菌属于芽孢杆菌属,占总量的29%;19株菌属于节杆菌属,占总量的11%;这3个属菌株合计占采集资源总量的76%。随地域和作物种类分布的特点是芽孢杆菌和类芽孢杆菌两个属的菌种资源具有很强的地域广泛性和作物广泛性,即从采自全国各地、各种作物的土壤样品几乎都可以分离到这两类菌种。这个研究结果对微生物肥料菌种选育和生产应用具有指导意义。

关 键 词:非共生    生物固氮    微生物资源    系统发育
收稿时间:2008-10-6

Collection and investigation on asymbiotic nitrogen-fixing microbial resources from 13 provinces over China
SUN Jian-guang,XU Jing,HU Hai-yan,ZHANG Yan-chun,LIU Jun,WANG Wen-bo,SUN Yan-hua.Collection and investigation on asymbiotic nitrogen-fixing microbial resources from 13 provinces over China[J].Plant Nutrition and Fertilizer Science,2009,15(6):1450-1465.
Authors:SUN Jian-guang  XU Jing  HU Hai-yan  ZHANG Yan-chun  LIU Jun  WANG Wen-bo  SUN Yan-hua
Institution:1.Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China
Abstract:One hundred and eighty one asymbiotic nitrogen-fixing bacterial strains were isolated from 70 soil samples collected from 13 provinces over China. Results based on the determination of morphology, physiology and chemical properties, together with 16S rDNA sequencing, indicated that these strains belong to 66 species of 24 genera, which account for about a half of currently reported genera of asymbiotic nitrogen-fixing bacteria. Taxonomic distribution of these strains was relative converging, 65 strains belongs to Paenibacillus, 52 strains belongs to Bacillus and 19 strains belongs to Arthrobacter, which accounts for 36%, 29% and 11% of gross resource, respectively. Strains of these 3 groups make up 76% of gross resource. Strains of Paenibacillus and Bacillus showed significant diversity on the distribution regarding to geography and crops. Namely strains of these 2 groups could be isolated from almost every sample collected from root area of crops all over China.
Keywords:asymbiotic  biological nitrogen fixation  microbial resources  phylogeny
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