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一株抗药用白菊枯萎病生防菌的分离与生防效应研究
引用本文:杨娜,沈其荣,徐阳春.一株抗药用白菊枯萎病生防菌的分离与生防效应研究[J].植物营养与肥料学报,2011,17(3):731-736.
作者姓名:杨娜  沈其荣  徐阳春
作者单位:1.南京农业大学资源与环境学院,江苏南京 1.0pt;mso-ansi-language: EN-US;mso-fareast-language:
基金项目:农业部行业科技专项,国家自然科学基金,江苏省科技支撑项目
摘    要:从生长健康的药菊根际土壤分离筛选获得一株对其连作土传枯萎病致病菌(尖孢镰刀菌菊专化型)具有较强拮抗作用的细菌a4,并通过盆钵和田间小区试验研究其生防效果。结果表明,拮抗菌a4对药菊枯萎病病原菌的抑制率达71%。盆栽试验,施用a4二次发酵的生物有机肥(CK+BOF1),未发生药菊枯萎病,而对照植株则完全发病;对照植株根际土壤尖孢镰刀菌的数量为CK+BOF1处理的6.5倍。田间小区试验,苗床育苗和移栽时都施用BOF并加灌a4发酵液的处理(n+F+a4),根际土和非根际土中病原菌的数量分别为2.0103和2.8103 cfu/g,而对照则分别为1.4104和1.2104 cfu/g。n+F+a4处理生长120 d的药菊生物量(干重)为对照的4.47倍,生防效果达93.2%。采用苗床育苗和移栽时都施用高效拮抗菌的方法,能有效防止枯萎病发生,促进药菊植株生长,提高产量。

关 键 词:药菊    枯萎病    生物肥料    生防
收稿时间:2010-10-08

Isolation and biological control of an antagonistic bacteria against medicinal chrysanthemum fusarium wilt
YANG Na,SHEN Qi-rong,XU Yang-chun.Isolation and biological control of an antagonistic bacteria against medicinal chrysanthemum fusarium wilt[J].Plant Nutrition and Fertilizer Science,2011,17(3):731-736.
Authors:YANG Na  SHEN Qi-rong  XU Yang-chun
Institution:1.College of Resources and Environmental Science,Nanjing Agricultural University,Nanjing 210095,China
Abstract:In this experiment,one of antagonistic bacteria named a4 was isolated from the soil which collected from the rhizosphere of regular growing medicinal chrysanthemum,its resistance against the pathogen(Fusarium oxysporum f.sp.Chrysanthemi) was screened respectively by the improved confront culture method,and its biocontrol effect on the medicinal chrysanthemum fusarium wilt was examined by the pot experiment and the field plot trials.Results showed: 1)The inhibition rate of a4 against the pathogen was 71%.2) ...
Keywords:medicinal chrysanthemum  medicinal chrysanthemum fusarium wilt  bio-organic fertilizer  biocontrol  
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