| 蛇床子素对小麦赤霉病菌葡萄糖、钙吸收和三磷酸腺苷酶活性的抑制 |
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| 引用本文： | 沈寿国,石志琦,徐朗莱,范永坚.蛇床子素对小麦赤霉病菌葡萄糖、钙吸收和三磷酸腺苷酶活性的抑制[J].农药学学报,2005,7(2):135-139. |
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| 作者姓名： | 沈寿国 石志琦 徐朗莱 范永坚 |
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| 作者单位： | 1.江苏省农业科学院 食品安全研究中心,江苏 南京 210014 |
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| 基金项目： | 江苏省自然科学基金(BK2002-128)，国家高技术研究发展计划(“863”计划)(2002AA244041). |
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| 摘 要： | 蛇床子素可抑制小麦赤霉病菌菌丝生长干重;含有14C标记的葡萄糖培养基中培养小麦赤霉病菌,以100 μg/mL蛇床子素处理,其菌体内14C放射强度比对照降低43％,表明蛇床子素能抑制小麦赤霉病菌对葡萄糖的吸收;以100 μg/mL蛇床子素处理小麦赤霉病菌12 h,菌体内总钙含量仅为对照的60%,表明蛇床子素能抑制菌体对钙的吸收;蛇床子素处理对β-1,6-葡聚糖酶活性没有显著影响;离体条件下,蛇床子素能抑制小麦赤霉病菌三磷酸腺苷(ATP)酶活性。
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| 关 键 词： | 蛇床子素 小麦赤霉病菌 葡萄糖吸收 钙吸收 三磷酸腺苷酶活性 |
| 文章编号： | 1008-7303(2005)02-0135-05 |
| 收稿时间： | 2004/12/1 0:00:00 |
| 修稿时间： | 2004年12月1日 |
Inhibition of Osthol on the Uptake of Glucose, Calcium and ATPases Activity in Fusarium graminearum |
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| SHEN Shou-guo,SHI Zhi-qi,XU Lang-lai and FAN Yong-jian.Inhibition of Osthol on the Uptake of Glucose, Calcium and ATPases Activity in Fusarium graminearum [J].Chinese Journal of Pesticide Science,2005,7(2):135-139. |
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| Authors: | SHEN Shou-guo SHI Zhi-qi XU Lang-lai and FAN Yong-jian |
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| Institution: | 1.Food Safety Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China2.Department of Biology Science, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Dry biomass of Fusarium graminearum was inhibited by osthol.When F. graminearum bred with 14C-glucose was treated with 100 μg/mL osthol, the radio activity decreased by 43%. When F. graminearum was treated with 100 μg/mL osthol for 12 h, calcium in F. graminearum decreased by 40%. It suggested that osthol inhibited hyphae uptake of calcium as well as glucose. PAGE indicated that osthol also inhibited the activity of ATPase in vitro, but cell wall lysis-related β-1,6-glucanase activity had not varied compared with contol. |
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| Keywords: | osthol F graminearum ~(14)C-glucose the uptake of calcium ATPase activity |
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