大白猪MUC13基因多态性与生长、肉质性状的相关性 |
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引用本文: | 刘亚轩,肖石军,郭长明,陈福珍,林金玉,陈晓燕,沈金清,杨锋,林长光.大白猪MUC13基因多态性与生长、肉质性状的相关性[J].福建农业大学学报,2014(3):295-298. |
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作者姓名: | 刘亚轩 肖石军 郭长明 陈福珍 林金玉 陈晓燕 沈金清 杨锋 林长光 |
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作者单位: | [1]福建光华百斯特生态农牧发展有限公司,福建尤溪365106 [2]江西农业大学动物生物技术国家重点实验室培育基地,江西南昌330045 [3]福建省农业科学院畜牧兽医研究所,福建福州350013 |
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基金项目: | 国家生猪产业技术体系建设项目(CARS-36);福建省科技厅区域重大专项(2011N3022);福建省科技重大专项(2012NZ0003-3);福建省生猪产业技术体系项目(闽农科教[2009]200号). |
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摘 要: | 选择对仔猪腹泻有显著影响的产肠毒索大肠杆菌(ETEC)F4ac的受体基因MUC13为研究对象,采用PCR-SNaPshot判型与常规育种技术相结合的方法,对544头大白猪核心育种群进行基因判定及基因型与生长、肉质性状相关性的研究.结果表明,大白猪MUC13基因型分布大小为:腹泻易感杂合基因型GA〉腹泻抗性纯合基因型GG〉腹泻易感纯合基因型从,腹泻抗性有利基因G的等位基因频率为0.669,腹泻易感不利基因A的等位基因频率为O.331.3种基因型个体达100kg日龄及其估计育种值(EBV)、达100kg背膘厚及其EBV的大小为:从〉GA〉GG;眼肌面积、肌内脂肪含量和估计瘦肉率的大小为:GG〉GA〉AA,差异不显著(P〉0.05).可见,在种猪选育上,逐代选择带有G等位基因的个体,建立抗性纯合GG基因型个体的种猪核心群,即可降低仔猪腹泻率,又不影响生长和肉质的性状.
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关 键 词: | 大白猪 MUC13基因 生长性状 肉质性状 |
The polymorphism of MUCl3 gene of large white pig and its correlation with the traits of growth and meat quality |
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Institution: | LIU Ya-xuan, XIAO Shi-jun, GUO Chang-ming, CHEN Fu-zhen, LIN Jin-yu, CHEN Xiao-yan, SHEN Jin-qing, YANG Feng, LIN Chang-guan( 1. Fujian Guanghua BEST Eco-agrlculture and Animal Husbandry Development Co. , Ltd. , Youxi, Fujian 365106, China; 2. Candidate of National Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; 3. Institute of Animal Science and Veterinary Medicine, Academy of Agricultural Sciences of Fujian Province, Fuzhou, Fujian 350013, China) |
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Abstract: | With 544 nucleus breeding large white pigs as material, PCR-SNaPshot and conventional breeding test was used to deter-mine the genotypes, and the correlations between genotypes of MUCl3 and the traits of growth and meat quality were analyzed. Theresult showed that the frequencies of 3 genotypes were GA 〉 GG 〉 A.A, G allele frequency was 0.669, A allele frequency was 0.331.The ages of 100 kg body weight and its estimated breeding value (EBV) of the 3 genotypes were AA 〉 GA 〉 GG, as well as the backfat thickness of 100 kg body weight and its EBV. Eye muscle area, intramuscular fat content, and estimated lean meat percentage ofthe 3 genotypes were GG 〉GA 〉AA , which were not significant (P 〉0.05). Therefore, in the selection process of pig breeding, theindividuah with allele G should be selected generation-by-generation for reducing diarrhea rate of piglets, establishing a favorable gen-otype of MUCl3 pure line core group, which would not affect the growth rate and meat quality traits in the breeding large white pigs. |
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Keywords: | large white pig MUCl3 gene polymorphism growth trait meat trait |
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