| 多花水仙HMGS基因的克隆 |
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| 引用本文： | 李科,何炎森,陈晓静.多花水仙HMGS基因的克隆[J].福建农业大学学报,2014(3):289-294. |
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| 作者姓名： | 李科 何炎森 陈晓静 |
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| 作者单位： | [1]福建农林大学园艺学院、园艺植物遗传育种研究所,福建福州350002 [2]福建省农业科学院闽台园艺研究中心,福建漳州363005 |
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| 基金项目： | 福建省高校产学合作科技重大项目（2011N51010043）. |
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| 摘 要： | 以多花水仙花瓣抑制消减杂交文库获得的羟甲基戊二酰辅酶A合酶（HMGS）基因片段为基础，采用cDNA末端快速扩增（RACE）技术从黄花水仙2号和金盏银台中各克隆一条HMGS基因，两基因均含有一个1413bp的开放阅读框，编码470个氨基酸，但存在2个氨基酸差异．氨基酸序列分析表明：黄花水仙2号与葡萄、大豆、蓖麻和玉米HMGS基因的氨基酸相似系数分别为83％、82％、82％和81％．以水仙Actin基因为内参基因，采用荧光定量PCR方法分析HMGS基因在两品种的表达水平，结果表明：两品种HMGS基因的表达水平差异并不明显．
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| 关 键 词： | 多花水仙 羟甲基戊二酰辅酶A合酶（HMGS） 克隆 |
Cloning of HMGS gene from flowers of Narcissus tazetta |
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| LIKe,HE Yan-sen,CHEN Xiao-jing.Cloning of HMGS gene from flowers of Narcissus tazetta[J].Journal of Fujian Agricultural University,2014(3):289-294. |
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| Authors: | LIKe HE Yan-sen CHEN Xiao-jing |
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| Institution: | 1. College of Horticulture/Institute of Genetics ＆ Breeding in Horticultural Plants, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. Mintai Horticulture Research Center, Fujian Academy of Agrieultural Sciences, Zhangzhou, Fujian 363005, China) |
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| Abstract: | Two 3-hydroxy-3-methylglutaryl-CoA synthase （HMGS） genes were cloned from Narcissus tazetta var.Huanghuashuixian H and Jinzhanyintai by rapid amplification of cDNA ends （RACE）. The open reading frame （ORF） in both ofthe gene encompassed 1413 bp encoding a polypeptide of 470 amino acids, and there are two amino acids different between them.Sequencing analysis showed that the amino acid sequence of N. tazetta was 83%, 82%, 82%, 81% homologous with HMGS genesfrom Vitis vinifera, Glycine max, Ricinus communis, Zea may respectively. The relative expression levels of HMGS at two varietieswere analyzed by qRT-PCR and Actin was used as reference gene. The qRT-PCR results showed that HMGS relative expression lev-els at the two varieties are not obviously differential. |
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| Keywords: | Narcissus tazetta 3-hydroxy-3-methylglutaryl-CoA synthase （HMGS） clone |
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