莆田黑猪H-FABP基因单核苷酸多态性分析

利用PcR．SSCP技术，分析莆田黑猪心脏型脂肪酸结合蛋白（H-FABP）基因的多态性．结果表明：在H-FABP基因5＇-调控区检测到两个多态性位点（G7T和C11A），由两个等位基因控制，定义为A和B，基因频率分别为0．740和0．260；在外显子2处存在3个多态性位点（A2C、T62C和T65C），由两个等位基因控制，定义为c和D，基因频率分别为0．164和0．836．群体遗传分析表明，H-FABP基因5＇-调控区属中度多态（0．25〈PIC〈0．5），外显子2区属低度多态（PIC〈0．25）．）c2适合性检验表明，两位点均处于Hardy-Weinberg平衡状态（P〉0．05）．将莆田黑猪H-FABP基因外显子2的核苷酸序列与GenBank中野猪核苷酸序列进行同源性比对，同源性为100％，而与牦牛、驴、人、大鼠、鸡和斑马鱼的同源性分别为94．0％、92．4％、87．8％、82．4％,77．9％和67．2％．

Single nucleotide polymorphisms of H.FABP gene in Putian Black pig

Polymorphisms of heart fatty acid-binding protein （H-FABP） gene in Putian Black pigs were detected by PCR-SSCP method. The results showed that two SNPs （ G7T and CllA） were detected in 5＇ - regnlatory region, and two alleles, designated as A and B, were found. The allele frequencies were 0.740 and 0.260 respectively. Three SNPs （A2C,T62C and T65C） were detec- ted in exord, and two alleles, designated as C and D, were found. The allele frequencies were 0.164 and 0.836 respectively. Sta- tistical analysis revealed an intermediate PIC in 5＇ - regulatory region （0.25 〈 PIC 〈 0.5 ） but a low PIC in exon2 （ PIC 〈 0.25 ）. A chl-square analysis suggested that the allele were in Hardy-Weinberg equilibrium （P〉0.05）. The nucleotide sequences of exon2 in Putian Black pig show 100% homology with the same part of Sus scrofa in GenBank, and with Bos gmnniens, Equus asinus, Homo sapiens, Rawa norregicus, Gallus gallus and Danio redo was 94.0%, 92.4%, 87.8%, 82.4%, 77.9% and 67.2% respectively.