IgM-ELISA法用于日本血吸虫病早期诊断初探

目的 验证血清特异性IgM抗体检测对急性日本血吸虫病的早期诊断价值。方法 采用IgM-ELISA方法检测感染血吸虫的小鼠和急性血吸虫病人血清中的特异性IgM抗体水平，与IgG—ELISA相比较，并与急性血吸虫病人的流行病学调查资料相比对。结果 感染4周时小鼠血清中特异性IgM抗体水平显著升高，5周时阳性检出率达100％，比IgG-ELISA提早2-3周。检测急性血吸虫病人血清阳性率达100％，IgG—ELISA为91．4％。急血病人接触疫水后5周，特异性IgM抗体水平明显高于IgG。7周时两者水平相近，8周时IgG抗体水平超过IgM。结论 IgM—ELISA具有早期诊断急性血吸虫病的价值.

EVALUATION OF EARLY DIAGNOSIS VALUE OF IgM-ELISA FOR SCHISTOSOMIASIS JAPONICA

To evaluate the early diagnosis value for schistosomiasis japonica with IgM-ELISA,the antibodies specific to SEA from schistosome infected mice or acute schistosomiasis patient sera before and post-infection were detected using IgM-ELISA or IgG-ELISA.The IgM antibody increasing remarkably at day 28 post-inffection. All mice were positive of IgM antibody at day 35 post-infection with IgM-ELISA.But with IgG-ELISA all mice were positive at day 49 post-infection.All acute schistosomiasis patients' sera were positive with IgM-ELISA, but 3 acute schistomiasis patients' sera were negative with IgG-ELISA.The IgM antibody was largely more than IgG antibody before five weeks post-infection,and as more as IgG antibody within following two weeks,but less than IgG antibody after eight weeks post-infection.The results indicated that the IgM-ELISA method has a high value for early diagnosis of schistosomiasis japonica.