茶树无菌播种建立植株再生体系

为提高茶树的繁殖速率,用近成熟的茶树种子以无菌播种方式获得试管苗,再以其茎段为外植体,建立了茶树高效植株再生体系。结果表明,剥除内外种皮的茶树种子发芽率为90%,较保留种皮的完全种子增加了13个百分点;茶树种子胚龄与发芽势、发芽率呈正相关,与萌发时间呈负相关;诱导茶树种子萌发适宜培养基为MS+6-BA 3.0mg/L+IBA 2.0mg/L+YE 200mg/L或改良ER+6-BA 2.5mg/L+NAA 0.2mg/L;在改良ER+6-BA 1.5mg/L+NAA 0.2mg/L继代增殖培养基中培养,带芽组织切块的适宜增殖周期为39d,增殖倍数5.4;壮苗、诱导根形成的适宜培养基为1/2改良ER+IBA 0.10mg/L,发根率达80%。

Establishment of Plant Regeneration System of Tea Trees by Asepsis Sowing

To improve the reproduction rate of tea trees,using the stem of plantlet as explant,which was obtained by asepsis sowing with the nearly mature tea seeds,the optimum regeneration system of tea trees was established.The experimental results showed that the germination rate of tea seeds whose endotesta and exopleura were stripped out was 90%,increased by 13 percentage points compared with the whole seeds.The embryo age of tea seeds had positive correlation with germination energy and germination percentage,but was negatively related to the germination time.To induce tea seed germination,the optimum medium was composed of MS+6-BA 3.0 mg/L+IBA 2.0 mg/L+YE 200 mg/L or ER+6-BA 2.5 mg/L+NAA 0.2 mg/L.The tissue slicing of bud indicated that the suitable proliferation cycle was 39 days with proliferation multiple of 5.4 in the modified subculture medium(ER+6-BA 1.5 mg/L+NAA 0.2 mg/L).In addition,the optimum medium for making seedling strong and inducing root formation was modified 1/2 ER+IBA 0.10 mg/L,leading to 80% rooting rate.