| 蜂斗叶组培快繁体系的建立 |
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| 引用本文： | 冷春玲,刘丹梅.蜂斗叶组培快繁体系的建立[J].安徽农业科学,2007,35(2):373-373,375. |
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| 作者姓名： | 冷春玲 刘丹梅 |
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| 作者单位： | 辽东学院农业与环境学院生物系,辽宁丹东,118003;辽东学院农业与环境学院生物系,辽宁丹东,118003 |
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| 摘 要： | 对蜂斗叶的组培条件进行了较系统的研究.结果表明,以蜂斗叶的叶柄切段为外植体,在MS+6-BA 2mg/L培养基上可诱导出胚性愈伤组织并分化出丛生芽;芽丛在MS+6-BA1 mg/L的继代培养基上,月繁殖系数可达3倍以上;健壮芽在1/2 MS+NAA 0.1 mg/L+活性炭1 mg/L的生根培养基上生根率为100%.
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| 关 键 词： | 蜂斗叶 组织培养 |
| 文章编号： | 0517-6611（2007）02-00373-01 |
| 修稿时间： | 2006-05-16 |
Establishment of Rapid Propagation in Tissue Culture of Petasites Japonicus |
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| LENG Chun-ling et al.Establishment of Rapid Propagation in Tissue Culture of Petasites Japonicus[J].Journal of Anhui Agricultural Sciences,2007,35(2):373-373,375. |
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| Authors: | LENG Chun-ling |
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| Institution: | Agrieuhure and Environment College, Liaodong University, Dandong, Liaoning 118003 |
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| Abstract: | In this paper,the condition of tissue culture and rapid propagation of Petasites Japonicus was studied.The results showed that cultivated on MS 2 mg/L BA media,petioles produced callus and shoots;when shoots were cultivated on MS 1mg/L BA media,the reproduce coefficient was more than 3 times;and when the shoots were cultivated on 1/2 MS 0.2 mg/L NAA 1 mg/L active carbon media,the rhizogenesis rate was 100 %. |
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| Keywords: | Petasites japonicus Miq Tissue culture |
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