影响盾叶薯蓣微块茎形成因素的研究

研究了离体条件下影响盾叶薯蓣微块茎形成的各种因素。结果表明:6.0或8.0 mg/L BA和1.0 mg/L KT组合适宜于微块茎的形成;提高培养基中蔗糖浓度有利于微块茎形成,在蔗糖浓度为80 g/L的培养基上,微块茎发生频率高达77.3%,平均重量达367.6 mg;黑暗处理有利于微块茎发生,20 d黑暗处理使微块茎发生频率达81.2%,平均重量为386.2 mg,且微块茎质量较好。这些微块茎在离体或田间均能较好的萌发、生长。

Factors Affecting Microtuber Formation of Dioscorea zingiberensis in vitro

Several factors affecting microtuber formation of Dioscorea zingiberensis C.H Wrihgt in vitro were studied here.The result showed that the combination of 6.0 or 8.0 mg/L BA and 1.0 mg/L KT was optimal for the formation of microtuber.The enhancement of sucrose in the medium favored the formation of microtuber,on the medium containing 80 g/L sucrose,microtuber occurred at frequency of 77.3% and their weight averaged 367.6 mg.Darkness treatment could improve microtuber formation,darkness treatment for 20 days could increase the frequency of microtuber formation to 81.2% and mean weight to 386.2 mg,and the microtuber had better quality.These microtubers could germinate and grow well in vitro or in field.