Assessing the genetic diversity of Portuguese maize germplasm using microsatellite markers |
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Authors: | MC Vaz Patto Z Satovic S Pêgo P Fevereiro |
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Institution: | 1. Instituto de Tecnologia Química e Biológica (ITQB), Plant Cell Biotechnology Lab, Apart. 127, 2781-901, Oeiras, Portugal 2. Faculty of Agriculture, Department of Seed Science and Technology, University of Zagreb, Svetosimunska 25, 10000, Zagreb, Croatia 3. Esta??o Agronómica Nacional (EAN), Av. República, 2784-505, Oeiras, Portugal
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Abstract: | A collection of Portuguese maize accessions representing a valuable source of genes for introduction into modern cultivars
is stored at the Portuguese Plant Germplasm Bank (Banco Português de Germoplasma Vegetal—BPGV). To assess genetic diversity
among inbreds, microsatellite analysis was carried out for 54 inbred lines representing the diversity of Portuguese dent and
flint maize germplasm. Fifty American and other European elite inbreds were also analysed for comparison. Fifteen microsatellite
loci distributed throughout the maize genome were chosen based on their repeat unit and base composition. A total of 80 alleles
were detected with an average allele number of 5.33 per locus. Polymorphism information content (PIC) values and observed
genetic distances showed the existence of large variability among inbreds. Cluster analysis indicated that almost all of the
inbreds could be distinguished from each other and Portuguese inbreds were present in all clusters formed. These associations
were consistent with the known pedigree records of the inbreds, confirming a mixed origin of Portuguese materials. Comparative
analysis of microsatellite diversity among groups was established according to important traits for both breeding and line
identification. This revealed that, although most of the genetic diversity (>95%) was attributable to differences among inbreds
of different groups, the existence of phenotypic differentiation in endosperm colour, kernel type and cob colour could be
suggested for grouping. These findings support the joint use of molecular and morphological traits in management of the germplasm
collection. In this study, SSR markers proved to be effective to characterise and identify maize inbred lines, and demonstrate
associations among them.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | AMOVA genetic diversity maize microsatellite Zea mays L |
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