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| 黑麦草抗冻蛋白基因的cDNA克隆及序列分析 | |
| 引用本文： | 邓顺阳,张党权,樊绍刚,谷振军,黄青云,明付焕,祝全东,郭林林. 黑麦草抗冻蛋白基因的cDNA克隆及序列分析[J]. 中南林业科技大学学报(自然科学版), 2010, 30(1) |
| 作者姓名： | 邓顺阳 张党权 樊绍刚 谷振军 黄青云 明付焕 祝全东 郭林林 |
| 作者单位： | 中南林业科技大学,经济林育种与栽培国家林业局重点实验室,湖南,长沙,410004 |
| 基金项目： | 国家自然科学基金项目，湖南省自然科学基金项目，中南林业科技大学青年基金重点项目 |
| 摘 要： | 对多年生黑麦草进行冷胁迫处理后,提取叶片总RNA,采用RT-PCR法获得了LpAFP成熟蛋白的cDNA克隆。序列分析结果表明,该成熟蛋白的开放读码框(ORF)长度为357 bp,编码一条由118个氨基酸组成的多肽。生物信息学分析结果表明,黑麦草成熟抗冻蛋白与其他植物抗冻蛋白的同源性较低,与鱼类抗冻蛋白和昆虫抗冻蛋白则没有同源性。 |
| 关 键 词： | 生物技术 黑麦草 抗冻蛋白 cDNA克隆 开放读码框 生物信息学 |
cDNA cloning and sequence analysis of antifreeze protein gene of Lolium perenne |
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| DENG Shun-yang,ZHANG Dang-quan,FAN Shao-gang,GU Zhen-jun,HUANG Qing-yun,MING Fu-huan,ZHU Quan-dong,GUO Lin-lin. cDNA cloning and sequence analysis of antifreeze protein gene of Lolium perenne[J]. , 2010, 30(1) | |
| Authors: | DENG Shun-yang ZHANG Dang-quan FAN Shao-gang GU Zhen-jun HUANG Qing-yun MING Fu-huan ZHU Quan-dong GUO Lin-lin |
| Affiliation: | DENG Shun-yang,ZHANG Dang-quan,FAN Shao-gang,GU Zhen-jun,HUANG Qing-yun,MING Fu-huan,ZHU Quan-dong,GUO Lin-lin(The Key Lab.of Non-wood Forest Products of State Forestry Administration,Central South University of Forestry , Technology,Changsha 410004,Hunan,China) |
| Abstract: | Ryegrass(Lolium perenne) is an evergreen grass with strong cold resistance due to high active antifreeze protein(LpAFP).To clone the sequences of LpAFP,the total RNA of perennial ryegrass treated with cold stress was extracted.Then,the cDNA of mature LpAFP was obtained by RT-PCR.The sequence analysis result show that mature LpAFP contained 357 bp,which encoded 118 amino acids.The result of bioinformatics analysis reveales that ryegrass antifreeze protein has low homology to other plant antifreeze proteins,a... |
| Keywords: | biotechnology ryegrass antifreeze protein cDNA cloning open reading frame (ORF) bioinformatics |
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