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块茎与块根多酚氧化酶部分性质的比较
引用本文:雷晗枭,陈国伟,李晓辉,夏珊珊,吴美仙.块茎与块根多酚氧化酶部分性质的比较[J].安徽农业科学,2014(33):11617-11618.
作者姓名:雷晗枭  陈国伟  李晓辉  夏珊珊  吴美仙
作者单位:浙江海洋学院萧山科技学院,浙江杭州,311215;浙江海洋学院萧山科技学院,浙江杭州,311215;浙江海洋学院萧山科技学院,浙江杭州,311215;浙江海洋学院萧山科技学院,浙江杭州,311215;浙江海洋学院萧山科技学院,浙江杭州,311215
基金项目:2013年度浙江海洋学院大学生科技创新项目(自然科学类),浙江省教育厅科研项目
摘    要:目的]为高职学生实验《多酚氧化酶的制备和性质研究》筛选合适的实验材料.方法]选取马铃薯和紫薯比较研究块茎与块根中多酚氧化酶(PPO)的活性、最适底物、最适温度等特性.结果]两者PPO最适底物均为邻苯二酚;马铃薯PPO的最适反应温度为35℃,紫薯PPO的最适反应温度为10 ℃.结论]经综合比较,该实验以马铃薯作为实验材料更为合适.

关 键 词:马铃薯  紫薯  多酚氧化酶  活性

Comparision of Some Natures of Polyphenol Oxidase in Stem Tuber and Root Tuber
LEI Han-xiao , CHEN Guo-wei , LI Xiao-hui , XIA Shan-shan , WU Mei-Xian.Comparision of Some Natures of Polyphenol Oxidase in Stem Tuber and Root Tuber[J].Journal of Anhui Agricultural Sciences,2014(33):11617-11618.
Authors:LEI Han-xiao  CHEN Guo-wei  LI Xiao-hui  XIA Shan-shan  WU Mei-Xian
Institution:(Xiaoshan College of Science and Technology, Zhejiang Ocean University, Hangzhou, Zhejiang 311215)
Abstract:Objective] The objective of this study was to choose ideal experiment material for the students' experiments-The Making and Natures Study of Polyphenol Oxidase(PPO) in higher vocational education.Method] The potato and purple potato were selected to study some natures of PPO,including its activity,optimum substrate and optimum reaction temperature.Result] Both of their optimum substrates were Catechol.The optimum reaction temperature of potato was 35 ℃,while that of purple potato was 10 ℃.Conclusion] After comprehensive comparison,potato was more ideal as experiment material than purple potato in this experiment.
Keywords:Potato  Purple potato  Polyphenol oxidase (PPO)  Activity
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