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The Effect of Total Anthocyanins Extracted From Sweet Cherry Cultivars on Carbonic Anhydrases and Antioxidant Activity

Authors:	Sonmez Fatih Gunesli Zuhal Demir Taki C?kr?kc? Kubra Ergun Adem Gencer Nahit Arslan Oktay

Institution:	1.Sakarya University of Applied Sciences, Pamukova Vocational High School, 54900, Sakarya, Turkey ;2.Horticulture Department, Faculty of Agriculture, Sakarya University of Applied Sciences, 54900, Sakarya, Turkey ;3.Department of Chemistry, Faculty of Art and Sciences, Balikesir University, 10145, Balikesir, Turkey ;

Abstract:	Total anthocyanins were extracted from eleven sweet cherry cultivars grown in Sakarya, Turkey. In vitro inhibition effects of extracted total anthocyanins on purified carbonic anhydrase I and II were investigated by using CO₂ as a substrate. The results showed that all extracted total anthocyanins inhibited the hCA I and hCA II enzyme activities. Among all cultivars, ‘Merton Premier’ was found to be the most active one against both hCA I (IC₅₀?=?0.83?µg/mL) and hCA II (IC₅₀?=?1.16?µg/mL). Additionally, the DPPH activities of the extracts were investigated as antioxidant properties. The results exhibited that ‘Churchill’ and ‘Merton Premier’ have high antioxidant activity with 74.102% and 73.503% DPPH activity, respectively.

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