一株仔猪肠道益生菌抗TGEV作用初探

为了分离并筛选出1株抗传染性胃肠炎病毒(TGEV)的益生菌菌株,研究采用形态观察、生化反应和16S rRNA鉴定等试验,并运用TCID50和MTT比色方法,在PK-15细胞水平上分别检测益生菌活菌、灭活菌体、菌体碎片及培养上清液在不同作用时间上对TGEV感染力和抑制率的影响。结果表明:从健康仔猪肠道内容物分离、鉴定出1株益生菌,属于德氏乳杆菌德氏亚种(暂命名为L1菌株),此菌株的活菌、灭活菌体、菌体碎片及培养上清液均有降低TGEV感染力和活性的作用,其中活菌降低TGEV感染力及对TGEV的抑制效果最明显,灭活菌体和培养上清液次之,菌体碎片最差。

Initial research on the effect of a probiotic strain in the piglet intestinal tract against TGEV

To isolate and screen a probiotic strain against TGEV,the experiments were carried out,including morphological observation,biochemical reaction and 16S rRNA identification.On the level of PK-15 cells,the effects of the infectivity and inhibition ratio of the living probiotic bacteria,inactived bacteria,bacteria fragments and culture supernatant liquids against TGEV experiment at different time were detected by using TCID50 and MTT.The result showed that a probiotic strain isolated from the intestinal content of healthy piglets was identificated as Lactobacillus delbrueckii subsp.delbrueckii(named L1).It proved that all different elements of the strain could reduce the infectivity and activity of TGEV,the living Lactobacillus was the best on the reducing infectivity of TGEV and the effect of inhibiting TGEV,the inactived bacteria and culture supernatant liquids came second,but the products from bacteria fragments was the worst.